Tran Nguyen Bao, Chang Ting-Kuo, Chi Nguyen Duong Phuong, Lai Kuan-Ying, Chen Hsien-Te, Fong Yi-Chin, Liaw Chih-Chuang, Tang Chih-Hsin
Graduate Institute of Biomedical Sciences, China Medical University, Taichung, Taiwan, 404.
Department of Medicine, Mackay Medical College, New Taipei, Taiwan, 252.
Int J Biol Sci. 2025 Jan 13;21(3):1144-1157. doi: 10.7150/ijbs.106827. eCollection 2025.
Chondrosarcoma is a rare type of bone cancer that develops in cartilage cells. In recent years, the incidence of chondrosarcomas has steadily increased worldwide. During the advanced stages, chondrosarcoma carries a significant risk of metastasis and exhibits resistance to both chemotherapy and radiation therapy. Hence, the development of potent treatments for chondrosarcoma is an urgent requirement. Ugonin V is a flavonoid compound that has been extracted from the plant (L.) Hook. This study examined the molecular therapeutic effects of ugonin V on chondrosarcoma metastasis. Analysis of the GSE30835 dataset, which consists of chondrosarcoma tissues and normal cartilage, revealed significant upregulation of three cathepsin proteases in chondrosarcoma, namely cathepsin (CTS) A, L, and V. Notably, ugonin V specifically suppressed cathepsin V mRNA expression. We also found that ugonin V strongly inhibits chondrosarcoma cell motility by regulating CTSV expression. In addition, through miRNA sequencing, we observed that ugonin V targets CTSV via miR-4799-5p to effectively suppress chondrosarcoma cell migration and invasion. Our and studies provide an initial investigation of the involvement of cathepsin V and miR-4799-5p in chondrosarcoma metastasis after ugonin V treatmen.
软骨肉瘤是一种在软骨细胞中发生的罕见骨癌类型。近年来，软骨肉瘤的发病率在全球范围内稳步上升。在晚期阶段，软骨肉瘤具有显著的转移风险，并且对化疗和放疗均表现出抗性。因此，开发有效的软骨肉瘤治疗方法是一项迫切需求。乌戈宁V是一种从植物（L.）胡克中提取的黄酮类化合物。本研究考察了乌戈宁V对软骨肉瘤转移的分子治疗作用。对由软骨肉瘤组织和正常软骨组成的GSE30835数据集的分析显示，软骨肉瘤中三种组织蛋白酶，即组织蛋白酶（CTS）A、L和V显著上调。值得注意的是，乌戈宁V特异性抑制组织蛋白酶V的mRNA表达。我们还发现，乌戈宁V通过调节CTSV表达强烈抑制软骨肉瘤细胞的运动性。此外，通过miRNA测序，我们观察到乌戈宁V通过miR-4799-5p靶向CTSV，以有效抑制软骨肉瘤细胞的迁移和侵袭。我们的[具体研究]和[具体研究]提供了对乌戈宁V治疗后组织蛋白酶V和miR-4799-5p参与软骨肉瘤转移的初步研究。
