Franco de Moraes Filho Leiser, Gomide Vilela de Sousa Franco Cláudia, Cruvinel Isaac David Leonardo, Louzada Ricardo Noguera, Pereira de Ávila Marcos, Magacho Leopoldo
Ophthalmology, Centro de Referência em Oftalmologia, Universidade Federal de Goiás, Goiânia, BRA.
Ophthalmology, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BRA.
Cureus. 2025 Jan 2;17(1):e76817. doi: 10.7759/cureus.76817. eCollection 2025 Jan.
This study compares the preparation time and the macroscopic integrity of Descemet membrane and endothelium keratoplasty (DMEK) grafts prepared using the modified submerged cornea using backgrounds away (SCUBA) technique compared to those prepared with a newly developed artificial chamber device specifically designed for DMEK preparation.
This is a prospective, comparative, randomized, and experimental study. Fifty corneas from 25 donors were used. The inclusion criteria were that both eyes were of the same donor, a scleral ring greater than 3 mm, and corneas unsuitable for clinical use. Preparation time and macroscopic tissue integrity were evaluated at the end of preparation.
The mean preparation time for both techniques was six minutes (p=0.2). The macroscopic integrity of the graft was similar between the inverted artificial chamber device and the modified SCUBA technique (p=1.00).
The upside-down artificial chamber operating with the upside-down cornea proved reproducible and macroscopically suitable for endothelial graft preparation for the DMEK technique.
本研究比较了使用改良的潜水员远离角膜（SCUBA）技术制备的Descemet膜和内皮角膜移植术（DMEK）移植物的制备时间和宏观完整性，与使用专门为DMEK制备而新开发的人工腔室装置制备的移植物进行比较。
这是一项前瞻性、比较性、随机化实验研究。使用了来自25名供体的50只角膜。纳入标准为双眼来自同一供体、巩膜环大于3mm且角膜不适合临床使用。在制备结束时评估制备时间和宏观组织完整性。
两种技术的平均制备时间均为6分钟（p=0.2）。倒置人工腔室装置和改良SCUBA技术之间移植物的宏观完整性相似（p=1.00）。
倒置人工腔室与倒置角膜配合操作被证明具有可重复性，并且在宏观上适用于DMEK技术的内皮移植物制备。
