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桂花甜米酒发酵过程中的酸度、糖分和酒精含量以及微生物群落动态

Acidity, sugar, and alcohol contents during the fermentation of Osmanthus-flavored sweet rice wine and microbial community dynamics.

作者信息

Tian Ping, Wan Jiaqiong, Yin Tuo, Liu Li, Ren Hongbing, Cai Hanbing, Liu Xiaozhen, Zhang Hanyao

机构信息

Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming, China.

R&D Department, Honghe Hongbin Food Co., Ltd., Jianshui, China.

出版信息

PeerJ. 2025 Jan 30;13:e18826. doi: 10.7717/peerj.18826. eCollection 2025.


DOI:10.7717/peerj.18826
PMID:39897497
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11787802/
Abstract

Sweet rice wine is a popular traditional Chinese rice wine widely loved by Chinese people for its high nutritional value. petals contain various nutrients and have good medicinal value. However, the dynamics of the sugar level, acidity, alcohol content, and microbial community during the fermentation of sweet rice wine have not been evaluated, which can lead to the unstable quality of flower sweet rice wine (OFSRW). In this study, the dynamic changes in sugar level, acidity, alcohol content, microbial community composition, and microbial metabolic pathways during traditional fermentation of OFSRW at four-time points-0 h (AG0), 24 h (AG24), 36 h (AG36), and 43 h (AG43)-were analyzed direct titration, total acid assays, alcoholometry, and high-throughput macrogenomic techniques. First, we found that bacteria were the dominant microorganisms in the early stage of OFSRW fermentation (AG0), fungi were the dominant microorganisms in the middle and late stages of fermentation (AG24 and AG36), and was the main fungal genus throughout fermentation. Acidity and total sugars increased with fermentation time, and alcohol was not detected until the end of fermentation. Diversity analysis revealed that the dominant species at the beginning of natural fermentation was , and became the dominant species as natural fermentation progressed. Metabolic pathway analysis revealed that energy production and conversion, carbohydrate transport, amino acid transport, and metabolic pathways were the most active metabolic pathways in the fermenter. These results provide a reference basis for changes in the microbial community during the fermentation of cinnamon-flavored sweet rice wine.

摘要

甜米酒是一种广受欢迎的中国传统米酒,因其高营养价值而深受中国人喜爱。花瓣含有多种营养成分,具有良好的药用价值。然而,甜米酒发酵过程中糖含量、酸度、酒精含量和微生物群落的动态变化尚未得到评估,这可能导致花香甜米酒(OFSRW)的质量不稳定。在本研究中,采用直接滴定法、总酸测定法、酒精测定法和高通量宏基因组技术,分析了OFSRW在0小时(AG0)、24小时(AG24)、36小时(AG36)和43小时(AG43)这四个时间点传统发酵过程中糖含量、酸度、酒精含量、微生物群落组成和微生物代谢途径的动态变化。首先,我们发现细菌是OFSRW发酵早期(AG0)的优势微生物,真菌是发酵中后期(AG24和AG36)的优势微生物,并且在整个发酵过程中是主要的真菌属。酸度和总糖含量随发酵时间增加,直到发酵结束才检测到酒精。多样性分析表明,自然发酵开始时的优势菌种是 ,随着自然发酵的进行 成为优势菌种。代谢途径分析表明,能量产生和转化、碳水化合物转运、氨基酸转运和代谢途径是发酵罐中最活跃的代谢途径。这些结果为肉桂味甜米酒发酵过程中微生物群落的变化提供了参考依据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5472/11787802/6fe18fa5a6f3/peerj-13-18826-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5472/11787802/bfad12aa20e2/peerj-13-18826-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5472/11787802/aabc1e7d5ee6/peerj-13-18826-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5472/11787802/1b192a3ed512/peerj-13-18826-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5472/11787802/f0cc2c0f24ba/peerj-13-18826-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5472/11787802/dc5fa2144846/peerj-13-18826-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5472/11787802/cd9a4cb72e98/peerj-13-18826-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5472/11787802/5bb0c2dd8144/peerj-13-18826-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5472/11787802/7bb3da094e69/peerj-13-18826-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5472/11787802/7da902bcd517/peerj-13-18826-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5472/11787802/6fe18fa5a6f3/peerj-13-18826-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5472/11787802/bfad12aa20e2/peerj-13-18826-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5472/11787802/aabc1e7d5ee6/peerj-13-18826-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5472/11787802/1b192a3ed512/peerj-13-18826-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5472/11787802/f0cc2c0f24ba/peerj-13-18826-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5472/11787802/dc5fa2144846/peerj-13-18826-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5472/11787802/cd9a4cb72e98/peerj-13-18826-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5472/11787802/5bb0c2dd8144/peerj-13-18826-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5472/11787802/7bb3da094e69/peerj-13-18826-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5472/11787802/7da902bcd517/peerj-13-18826-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5472/11787802/6fe18fa5a6f3/peerj-13-18826-g010.jpg

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Microbial Communities and Correlation between Microbiota and Volatile Compounds in Fermentation Starters of Chinese Sweet Rice Wine from Different Regions.

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[3]
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[4]
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[5]
Analysis of fungal dynamic changes in the natural fermentation broth of 'Hongyang' kiwifruit.

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[6]
Traditional uses, phytochemical constituents and pharmacological properties of Osmanthus fragrans: A review.

J Ethnopharmacol. 2022-7-15

[7]
Correlation between the microbial community and ethyl carbamate generated during Huzhou rice wine fermentation.

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[8]
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