Nencini Francesca, Bonari Alessandro, Ciullini Mannurita Sara, Mongia Alessandra, Romano Francesca, Garieri Maria, Russo Edda, Sastrucci Silvia, Marrani Graziella, Tonelli Martina, Salti Stefano, Funel Niccola, Amedei Amedeo, Dani Carlo, Fanelli Alessandra
General Laboratory, Careggi University Hospital, Florence, Italy.
Division of Neonatology and Neonatal Intensive Care Unit, Careggi University Hospital, Florence, Italy.
Pract Lab Med. 2025 Jan 14;44:e00453. doi: 10.1016/j.plabm.2025.e00453. eCollection 2025 Apr.
The use of a POCT (Point Of Care Test) could help in reducing the impact of pre-analytical errors in particular in challenging newborn samples.The study purpose is to compare the POCT Sight OLO® hematology analyzer, validated for >3 months patients, with the reference system Sysmex XN-9100™ in Neonatal Intensive Care Unit (NICU).
The two analyzers were compared through Passing-Bablok regression analysis and Bland-Altman plot.
We analyzed 65 blood samples, in detail 38 from adults and 27 from newborns.The regression analysis results performed in the newborn and adult patients showed a good agreement between the two instruments. The evaluation of the Bland-Altman plots showed comparable values of bias <10 % for the most of parameters.The evaluation of sample flags for the presence of distributional and morphological abnormalities showed a partial accordance between the two approaches, but the POCT exhibited good performance compared to the final report revised by the laboratory specialist.
The comparison of the two instruments demonstrated that they provide comparable blood counts, also in patients aged <3 months. The POCT allows having reliable analytical data and faster turning around time, particularly useful in NICU.
即时检验（POCT）的使用有助于减少分析前误差的影响，尤其是在处理具有挑战性的新生儿样本时。本研究的目的是在新生儿重症监护病房（NICU）中将已针对3个月以上患者验证的POCT Sight OLO®血液分析仪与Sysmex XN - 9100™参考系统进行比较。
通过Passing - Bablok回归分析和Bland - Altman图对这两种分析仪进行比较。
我们分析了65份血样，其中详细分析了38份成人血样和27份新生儿血样。在新生儿和成人患者中进行的回归分析结果显示，这两种仪器之间具有良好的一致性。对Bland - Altman图的评估表明，大多数参数的偏差值<10%，具有可比性。对存在分布和形态异常的样本标记的评估显示，两种方法部分一致，但与实验室专家修订的最终报告相比，POCT表现良好。
两种仪器的比较表明，它们在3个月以下的患者中也能提供可比的血细胞计数。POCT能够提供可靠的分析数据，且周转时间更快，这在NICU中尤为有用。
