Payne Hailey, Athans Christina, Wu Shiyong, Bahamondes Lorca Veronica
Honors Tutorial College Chemistry, Ohio University, Athens, Ohio, USA.
Honors Tutorial College Biological Sciences, Ohio University, Athens, Ohio, USA.
Photochem Photobiol. 2025 Feb 3. doi: 10.1111/php.14070.
There is a growing need for a skin model that combines the natural physiology of skin while reducing reliance on mice. Natural physiology is achieved by using fresh, intact skin explants sourced from living organisms such as humans or mice. This study focused on the standardization and characterization of an in vitro mouse skin explant model for investigating solar ultraviolet (sUV)-induced skin damage. We developed a protocol to use skin explants derived from the discarded tissue of mice after euthanasia. These explants consist of intact dermal and epidermal layers suspended in cell culture medium and maintained in vitro. To assess the viability of the skin explants, we evaluated tissue morphology (via hematoxylin and eosin [H&E] staining), viability markers, and DNA damage markers. Our ex vivo model preserves the key characteristics and physiological responses of in vivo skin for short incubation periods, while minimizing the use of mice. This model enables the study of DNA damage and repair, and it has broad applications, including studies on skin photoprotection, topical treatments, drug development, and cosmetics.
对一种既能结合皮肤自然生理特征又能减少对小鼠依赖的皮肤模型的需求日益增长。通过使用从人类或小鼠等生物体获取的新鲜、完整的皮肤外植体来实现自然生理特征。本研究聚焦于用于研究太阳紫外线（sUV）诱导的皮肤损伤的体外小鼠皮肤外植体模型的标准化和特性描述。我们制定了一个方案，使用安乐死后小鼠废弃组织来源的皮肤外植体。这些外植体由悬浮在细胞培养基中并在体外维持的完整真皮和表皮层组成。为了评估皮肤外植体的活力，我们评估了组织形态（通过苏木精和伊红[H&E]染色）、活力标志物和DNA损伤标志物。我们的体外模型在短培养期内保留了体内皮肤的关键特征和生理反应，同时尽量减少小鼠的使用。该模型能够研究DNA损伤和修复，并且具有广泛的应用，包括皮肤光保护、局部治疗、药物开发和化妆品研究。
