Nakamura Koki, Morishita Koki, Onda Nobuhiko, Sakai Ikuko, Matsumoto Shinya, Tamura Eri, Kouyama Yuta, Ogawa Yushi, Misawa Masashi, Hayashi Takemasa, Miyachi Hideyuki, Kudo Shin-Ei, Nemoto Tetsuo
Department of Biological Evaluation Analysis Technology, Olympus Medical Systems Corp., Tokyo, Japan.
Department of Optical Engineering, Olympus Medical Systems Corp., Tokyo, Japan.
Dig Endosc. 2025 Jun;37(6):659-669. doi: 10.1111/den.15000. Epub 2025 Feb 3.
To develop a procedure that matches magnifying endoscopic images with narrow-band imaging to 3D tissue structures using a tissue-clearing technique and to qualitatively and quantitatively analyze specified structures in gastrointestinal neoplasms.
Endoscopically resected formalin-fixed paraffin-embedded gastrointestinal tissues (three esophagus, four stomach, seven colon) were made transparent by ethyl cinnamate. They were then subjected to fluorescent staining of nuclei and blood vessels followed by 3D imaging using a confocal laser scanning microscope. A one-to-one correspondence between magnifying endoscopic and 3D reconstructed images was established using vessels and crypts with characteristic shapes as guides, and the depth and caliber of specified vessels were measured.
All tissues were optically cleared, which allowed 3D visualization of vascular structures and nuclei in all layers. In the esophagus, intraepithelial papillary capillary loops and subepithelial capillary networks were identified. In the upper part of the stomach, polygonal subepithelial capillary loops surrounding the pits were observed, while in the lower part, surface epithelium with ridge-like structures and coiled vessels were observed. A honeycomb pit structure and surrounding vascular structures were identified in the colon. Quantitative analysis showed the various contrasts of a single continuous vessel in the endoscopic image were due to different depths at which the vessel tortuously ran.
We established a procedure to allow one-to-one correspondence between magnifying endoscopic and 3D reconstructed images and to measure the depth and caliber of endoscopically visualized vessels of interest. This method is expected to improve endoscopic diagnosis and further the development of endoscopic imaging technologies.
开发一种利用组织透明化技术将放大内镜图像与窄带成像的三维组织结构进行匹配的方法，并对胃肠道肿瘤中的特定结构进行定性和定量分析。
将经内镜切除的福尔马林固定石蜡包埋的胃肠道组织（3例食管、4例胃、7例结肠）用肉桂酸乙酯使其透明。然后对细胞核和血管进行荧光染色，接着使用共聚焦激光扫描显微镜进行三维成像。以具有特征形状的血管和隐窝为导向，建立放大内镜图像与三维重建图像之间的一一对应关系，并测量特定血管的深度和管径。
所有组织均实现光学透明，从而能够对各层血管结构和细胞核进行三维可视化。在食管中，识别出上皮内乳头状毛细血管襻和上皮下毛细血管网。在胃的上部，观察到围绕胃小凹的多边形上皮下毛细血管襻，而在下部，观察到具有脊状结构和盘绕血管的表面上皮。在结肠中识别出蜂窝状小凹结构和周围的血管结构。定量分析表明，内镜图像中单个连续血管的各种对比度是由于血管曲折走行的不同深度所致。
我们建立了一种方法，可实现放大内镜图像与三维重建图像之间的一一对应，并测量内镜下可见的感兴趣血管的深度和管径。该方法有望改善内镜诊断并推动内镜成像技术的发展。
