Song Gaojie, Cheng Lingling, Liu Jun, Zhou Yu, Zhang Cuiling, Zong Yuping
Jiangxi Provincial Key Laboratory of Cell Precision Therapy, School of Basic Medical Sciences, Jiujiang University, Jiujiang, 332005, Jiangxi, P. R. China.
Department of Disease Prevention and Control, Navy 971 Hospital, Qingdao, P. R. China.
Sci Rep. 2025 Feb 3;15(1):4044. doi: 10.1038/s41598-025-88725-7.
This study aims to establish an animal model of monkeypox virus (MPXV) infection in dormice through intranasal inoculation. Male dormice aged 4-5 months were selected as experimental subjects and administered different titers of MPXV (10 PFU, 10 PFU, and 10 PFU, respectively) via nasal instillation. Within 14 days post-infection, clinical indicators such as survival rate, body weight changes, respiratory status, and mental state were continuously monitored. Additionally, tissue samples from the lungs, liver, spleen, and trachea of dormice from each group were collected on the 5th and 10th days for virus titer detection, and histopathological analysis was performed on lung samples collected on the 5th and 10th days. Dormice infected with MPXV exhibited typical symptoms such as appetite loss, continuous body weight reduction, aggravated respiratory difficulties, accompanied by lethargy, chills, and other clinical manifestations similar to human monkeypox infection. Virological tests further confirmed the distribution of MPXV in multiple vital organs of dormice, including the lungs, liver, spleen, and trachea, with particularly significant pathological damage observed in lung tissue. An MPXV infection model in dormice was successfully established through intranasal inoculation with a titer of 10 PFU MPXV, which can be used for studying the infection mechanism and pharmacology of MPXV.
本研究旨在通过鼻内接种建立睡鼠猴痘病毒（MPXV）感染的动物模型。选择4至5个月龄的雄性睡鼠作为实验对象，通过滴鼻给予不同滴度的MPXV（分别为10³ PFU、10⁴ PFU和10⁵ PFU）。在感染后14天内，持续监测存活率、体重变化、呼吸状态和精神状态等临床指标。此外，在第5天和第10天收集每组睡鼠的肺、肝、脾和气管组织样本用于病毒滴度检测，并对第5天和第10天收集的肺样本进行组织病理学分析。感染MPXV的睡鼠表现出典型症状，如食欲减退、体重持续减轻、呼吸困难加重，伴有嗜睡、寒战等类似于人类猴痘感染的临床表现。病毒学检测进一步证实了MPXV在睡鼠多个重要器官中的分布，包括肺、肝、脾和气管，其中肺组织观察到特别明显的病理损伤。通过鼻内接种10⁵ PFU滴度的MPXV成功建立了睡鼠MPXV感染模型，可用于研究MPXV的感染机制和药理学。
