Hassan Jawad, Javed Sarah, Ahmed Shakir, Javeed Waqas, Abid Madiha
Department of Hematology Laboratory, National Institute of Blood Disease and Bone Marrow Transplantation (NIBD), Karachi, Pakistan.
Department of Clinical and Molecular Cytogenetics, NIBD, Karachi, Pakistan.
Int J Lab Hematol. 2025 Jun;47(3):421-428. doi: 10.1111/ijlh.14434. Epub 2025 Feb 4.
To assess the significance of cell population data parameters (CPD) in dengue positive individuals.
A retrospective cross-sectional study was conducted at the National Institute of Blood Disease and Bone Marrow Transplantation (NIBD and BMT), Karachi, Pakistan from July 2022 to September 2022 (in a period of 3 months of peak dengue fever outbreak). Dengue fever is a viral infection that is transmitted from mosquitoes to humans. It is more prevalent in tropical and subtropical environments. A total of 389 individuals, who presented with febrile illness at the NIBD clinics, were screened for dengue and malaria with Complete Blood Count (CBC), Dengue nonstructural protein 1 (NS1) antigen test and Malaria Parasite Immunochromatographic test (MP-ICT). Whole blood samples were collected and analyzed for CBC on Sysmex XN hematology analyzers. All 65 CPD and standard CBC parameters were analyzed. Statistical analysis was performed using SPSS version 25.0. Descriptive analysis of all the parameters was performed and a p value < 0.001 was considered significant. Positive and negative correlation was also evaluated within the parameters to assess their significance. Furthermore, cut-off values of CPD parameters were evaluated plotting their receiver operating characteristic (ROC) curves.
Out of the 389 febrile patients, 137 were diagnosed as dengue-positive. Descriptive analysis for mean and median values of parameters revealed statistically significant difference for seven parameters (namely WBC, PLT-F, NEUT, LYMP, MONO, HFLC, and LY-WY) in the comparison of the two groups which were then further assessed for positive and negative correlation. Multivariate logistic regression analysis revealed High Fluorescence Lymphocyte Count (HFLC) to be the distinguishing parameter among dengue positive and negative cases. Compared to all the CPD parameters of our data set, the area under curve for lymphocytes cell size and the width of dispersion (LY-WZ) displayed a borderline value of 0.582.
Sysmex XN hematology analyzers can provide extensive information about CPD parameters, allowing for the prompt differentiation among febrile illnesses and dengue infection. HFLC and other significant parameters demonstrate promise as rapid, adjunctive diagnostic tools. Further research is needed to validate these findings and optimize the clinical utility of CPD parameters in dengue management.
评估细胞群体数据参数（CPD）在登革热阳性个体中的意义。
于2022年7月至2022年9月（登革热高热爆发的3个月期间）在巴基斯坦卡拉奇的国家血液疾病和骨髓移植研究所（NIBD和BMT）进行了一项回顾性横断面研究。登革热是一种由蚊子传播给人类的病毒感染。它在热带和亚热带环境中更为普遍。对在NIBD诊所出现发热疾病的389名个体进行了全血细胞计数（CBC）、登革热非结构蛋白1（NS1）抗原检测和疟原虫免疫层析检测（MP-ICT），以筛查登革热和疟疾。采集全血样本并在Sysmex XN血液分析仪上进行CBC分析。分析了所有65个CPD和标准CBC参数。使用SPSS 25.0版本进行统计分析。对所有参数进行描述性分析，p值<0.001被认为具有显著性。还评估了参数之间的正相关和负相关以评估其意义。此外，通过绘制接受者操作特征（ROC）曲线来评估CPD参数的临界值。
在389名发热患者中，137名被诊断为登革热阳性。参数均值和中位数的描述性分析显示，两组比较中有七个参数（即白细胞、血小板荧光强度、中性粒细胞、淋巴细胞、单核细胞、高荧光淋巴细胞计数和淋巴细胞宽度）存在统计学显著差异，随后对其进行正相关和负相关的进一步评估。多因素逻辑回归分析显示，高荧光淋巴细胞计数（HFLC）是登革热阳性和阴性病例之间的区分参数。与我们数据集中的所有CPD参数相比，淋巴细胞细胞大小和离散宽度（LY-WZ）的曲线下面积显示为临界值0.582。
Sysmex XN血液分析仪可以提供有关CPD参数的广泛信息，有助于快速区分发热疾病和登革热感染。HFLC和其他重要参数有望成为快速辅助诊断工具。需要进一步研究来验证这些发现并优化CPD参数在登革热管理中的临床应用。
