Isseroff A, Lancet D
J Neurosci Methods. 1985 Feb;12(4):265-75. doi: 10.1016/0165-0270(85)90011-1.
We describe a relatively inexpensive, yet versatile and powerful microcomputer-based image-analysis system, and its applications to processing of deoxyglucose autoradiographic data. Images are acquired via a video camera mounted on a light microscope or a light box, and digitized in 40 ms to 512 X 512 picture elements with 8-bit resolution (256 gray levels). The bit-mapped image analysis hardware can provide up to 256 colors for pseudo-color coding, and virtually instantaneous readout of brightness values for densitometry. The system is controlled by an 8-bit S-100 bus microcomputer, providing flexibility and ease of expansion. In addition to pseudo-color coding and densitometry, we have developed programs for averaging of successive sections, image subtraction and quantitative reconstruction of different planes of section from serial autoradiograms.
我们描述了一种相对廉价但功能多样且强大的基于微型计算机的图像分析系统，及其在处理脱氧葡萄糖放射自显影数据中的应用。图像通过安装在光学显微镜或灯箱上的摄像机获取，并在40毫秒内数字化为512×512像素，分辨率为8位（256个灰度级）。位图图像分析硬件可为伪彩色编码提供多达256种颜色，并能几乎即时读出用于光密度测定的亮度值。该系统由一台8位S - 100总线微型计算机控制，具有灵活性且易于扩展。除了伪彩色编码和光密度测定外，我们还开发了用于连续切片平均、图像减法以及从连续放射自显影片定量重建不同切片平面的程序。
