Li Linmei, Xue Bowei, Yan Shangbo, Shen Hui, Yang Yang, Fan Yiran, Zhang Ruiyang, Shen Weishou, Gao Nan
School of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, China.
School of 2011, Nanjing Tech University, Nanjing 211816, China.
J Appl Microbiol. 2025 Feb 3;136(2). doi: 10.1093/jambio/lxaf026.
Plant growth-promoting rhizobacteria (PGPR) improve microbial community structure, promote crop growth, and reduce greenhouse gas emissions in agricultural soils; however, the effects of PGPR fermentation on the growth and salt tolerance of tomato plants remain unclear. In this study, we aimed to investigate the effects of the PGPR Stutzerimonas stutzeri NRCB010 on the microbial communities, tomato growth, and nitrous oxide (N2O) emissions in saline soil by performing a greenhouse pot experiment.
The experiment was conducted under two soil salt concentrations (0 and 3 g kg-1 NaCl) and three treatments (LSFJ broth, NRCB010 cells, and NRCB010 culture). Both salt stress and NRCB010 treatments significantly affected the physicochemical properties and microbial community structure of tomato rhizosphere soil. Treatment with 3 g kg-1 NaCl significantly reduced the shoot and root dry weights of the plants compared with those of the control plants. Application of NRCB010 cells as well as that of culture promoted the growth of tomato seedlings and alleviated salt stress. The copy number changes in the nosZⅠ gene on day 3 and amoA gene on day 25 demonstrated that NRCB010 cells significantly reduced soil N2O emissions when treated with 0 g kg-1 NaCl. Furthermore, soil physicochemical properties, plant biomass, and soil microbial diversity were correlated with each other.
The results emphasize the enormous potential of S. stutzeri NRCB010 culture to resist abiotic stress, promote crop growth, and improve the rhizosphere soil microenvironment; however, its ability to decrease N2O emissions is constrained by soil salinity.
植物促生根际细菌（PGPR）可改善微生物群落结构，促进作物生长，并减少农业土壤中的温室气体排放；然而，PGPR发酵对番茄植株生长和耐盐性的影响仍不清楚。在本研究中，我们旨在通过进行温室盆栽试验，研究PGPR施氏假单胞菌NRCB010对盐渍土壤中微生物群落、番茄生长和一氧化二氮（N2O）排放的影响。
试验在两种土壤盐浓度（0和3 g kg-1 NaCl）和三种处理（LSFJ肉汤、NRCB010细胞和NRCB010培养物）下进行。盐胁迫和NRCB010处理均显著影响番茄根际土壤的理化性质和微生物群落结构。与对照植株相比，3 g kg-1 NaCl处理显著降低了植株地上部和根部的干重。施用NRCB010细胞以及培养物均促进了番茄幼苗的生长并缓解了盐胁迫。第3天nosZⅠ基因和第25天amoA基因的拷贝数变化表明，在0 g kg-1 NaCl处理时，NRCB010细胞显著降低了土壤N2O排放。此外，土壤理化性质、植物生物量和土壤微生物多样性之间相互关联。
结果强调了施氏假单胞菌NRCB010培养物在抵抗非生物胁迫、促进作物生长和改善根际土壤微环境方面的巨大潜力；然而，其降低N2O排放的能力受到土壤盐分的限制。
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