Liu Xixi, Wang Lifang, Li Guowei, Xu Chuantao, Li Yike, Peng Yong, Pu Jinping, Xie Yanfen, Chen Yunkun, Liu Zefan, Nian Fuzhao, Liu Di
College of Tobacco Science, Yunnan Agricultural University, Kunming, China.
Luzhou Branch Company, Sichuan Province Tobacco Company, Luzhou, China.
Front Microbiol. 2025 May 22;16:1601567. doi: 10.3389/fmicb.2025.1601567. eCollection 2025.
To further explore the biochar-soil-plant interaction mechanisms, rice husk biochar and root-promoting solutions were used to prepare biochar extracts and compound aqueous solutions, to study the regulation of biochar acid extract and its compound water agent on the growth of tobacco, as well as the effects of the main nutrient content (nitrogen, phosphorus, potassium), microbial abundance, and functional diversity in the rhizosphere soil of tobacco.
The results showed that the application of different concentrations of biochar acid extract and its compound aqueous enhanced tobacco plant dry matter accumulation, improved soil pH, increased available nitrogen and organic matter, and reduced potassium and phosphorus availability. Moreover, the effect of the compound water agent treatment group on soil nutrients was greater than that of the biochar acid extract treatment group. In terms of microorganisms, the application of biochar acid extract and its compound aqueous increased the relative abundance of , bacterial genus, , fungal genus, and functional bacteria such as chemoheterotrophs, aerobic chemoheterotrophs, and saprotrophs, promoting the complexity and interconnectivity of soil microbial networks.
In summary, the application of biochar acid extract and its compound aqueous solutions improve soil nutrients by enhancing the structure, diversity, and functional groups of the soil microbial community, thereby promoting tobacco growth. These results not only provide a theoretical basis for the interaction mechanism between biochar, soil, and tobacco, but also provide certain theoretical support for the development of biochar related fertilizers.
为进一步探究生物炭 - 土壤 - 植物的相互作用机制，利用稻壳生物炭和促根溶液制备生物炭提取物和复合水溶液，研究生物炭酸提取物及其复合水剂对烟草生长的调控作用，以及对烟草根际土壤中主要养分含量（氮、磷、钾）、微生物丰度和功能多样性的影响。
结果表明，不同浓度生物炭酸提取物及其复合水溶液的施用增强了烟草植株干物质积累，改善了土壤pH值，增加了有效氮和有机质含量，降低了钾和磷的有效性。此外，复合水剂处理组对土壤养分的影响大于生物炭酸提取物处理组。在微生物方面，生物炭酸提取物及其复合水溶液的施用增加了 细菌属、 真菌属以及化学异养菌、好氧化学异养菌和腐生菌等功能细菌的相对丰度，促进了土壤微生物网络的复杂性和连通性。
综上所述，生物炭酸提取物及其复合水溶液通过增强土壤微生物群落的结构、多样性和功能基团来改善土壤养分，从而促进烟草生长。这些结果不仅为生物炭、土壤和烟草之间的相互作用机制提供了理论依据，也为生物炭相关肥料的开发提供了一定的理论支持。
