Zulkeflee Nurul Nadiah, Mustapha Kamil Yasmin, Mashohor Syamsiah, Abu Bakar Muhammad Hafiz
Wireless and Photonics Network Research Centre (WiPNET), Universiti Putra Malaysia, UPM Serdang, 43400, Selangor, Malaysia; Department of Computer and Communication Systems Engineering, Faculty of Engineering, Universiti Putra Malaysia, UPM Serdang, 43400, Selangor, Malaysia.
Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, UPM Serdang, 43400, Selangor, Malaysia.
Biosens Bioelectron. 2025 Jun 1;277:117200. doi: 10.1016/j.bios.2025.117200. Epub 2025 Feb 4.
This work demonstrates a label-free dual-sensing biosensor utilizing cascaded single-mode tapered fiber (SMTF) for the concurrent detection of Dengue II envelope (E) and SARS-CoV-2 spike (S) proteins. Two fiber tapers of different dimensions were employed in-line, with each taper functionalized with specific complementary antibodies to the targeted antigens. The cascaded interferometric effect yielded composite spectral output that exhibited discrete response toward localized refractive index changes due to binding of antigens and antibodies. Consistent red shifts were observed with increasing concentration of the targeted analyte, which led to sensitivity values of 6.91 nm/nM for SMTF1 and 9.96 nm/nM for SMTF2, with a detection limit of 0.1 pM. This dual-sensing platform demonstrates high sensitivity and specificity, rapid response times, and the potential for integration into portable diagnostic devices, presenting it as a promising tool for point-of-care diagnostics and simultaneous detection of multiple disease biomarkers.
这项工作展示了一种无标记双传感生物传感器，它利用级联单模锥形光纤（SMTF）同时检测登革热II型包膜（E）蛋白和严重急性呼吸综合征冠状病毒2（SARS-CoV-2）刺突（S）蛋白。两个不同尺寸的光纤锥串联使用，每个光纤锥都用针对目标抗原的特异性互补抗体进行功能化修饰。级联干涉效应产生了复合光谱输出，该输出由于抗原和抗体的结合而对局部折射率变化表现出离散响应。随着目标分析物浓度的增加，观察到一致的红移，这导致SMTF1的灵敏度值为6.91 nm/nM，SMTF2的灵敏度值为9.96 nm/nM，检测限为0.1 pM。这个双传感平台展示了高灵敏度和特异性、快速响应时间以及集成到便携式诊断设备中的潜力，使其成为即时诊断和同时检测多种疾病生物标志物的有前途的工具。
