Hwang Hau-Hsuan, Huang Yu-Ting, Chien Pei-Ru, Huang Fan-Chen, Wu Chih-Lin, Chen Liang-Yu, Hung Shih-Hsun Walter, Pan I-Chun, Huang Chieh-Chen
Department of Life Sciences, National Chung Hsing University, No. 145, Xingda Road, Taichung, 402, Taiwan.
Innovation and Development Center of Sustainable Agriculture, National Chung Hsing University, Taichung, 402, Taiwan.
Bot Stud. 2025 Feb 4;66(1):7. doi: 10.1186/s40529-025-00453-3.
Due to global warming and gradual climate change, plants are subjected to a wide range of environmental stresses, adversely affecting plant growth and production worldwide. Plants have developed various mechanisms to overpower these abiotic stresses, including salt stress, drought, and high light intensity. Apart from their own defense strategies, plants can get help from the beneficial endophytic bacteria inside host plants and assist them in enduring severe growth conditions. A previously isolated plant endophytic bacteria, Burkholderia seminalis 869T2, from vetiver grass can produce auxin, synthesize siderophore, and solubilize phosphate. The B. seminalis 869T2 can colonize inside host plants and increase the growth of bananas, Arabidopsis, and several leafy vegetables.
We further demonstrated that different growth parameters of Arabidopsis and pak choi plants were significantly increased after inoculating the B. seminalis 869T2 under normal, salt, and drought stress conditions compared to the mock-inoculated plants. Both transcriptome analysis and quantitative real-time PCR results showed that expression levels of genes related to phytohormone signal transduction pathways, including auxin, gibberellin, cytokinin, and abscisic acid were altered in Arabidopsis plants after inoculated with the strain 869T2 under salt stress, in comparison to the mock-inoculated control with salt treatments. Furthermore, the accumulation levels of hydrogen peroxide (HO), electrolyte leakage (EL), and malondialdehyde (MDA) were lower in the 869T2-inoculated Arabidopsis and pak choi plants than in control plants under salt and drought stresses.
The plant endophytic bacterium strain B. seminalis 869T2 may affect various phytohormone responses and reduce oxidative stress damage to increase salt and drought stress tolerances of host plants.
由于全球变暖和气候逐渐变化，植物面临着广泛的环境胁迫，对全球植物生长和产量产生不利影响。植物已经发展出各种机制来克服这些非生物胁迫，包括盐胁迫、干旱和高光强度。除了自身的防御策略外，植物还可以从宿主植物体内的有益内生细菌获得帮助，协助它们在恶劣的生长条件下生存。先前从香根草中分离出的一种植物内生细菌伯克霍尔德氏菌869T2，能够产生生长素、合成铁载体并溶解磷酸盐。伯克霍尔德氏菌869T2可以在宿主植物体内定殖，并促进香蕉、拟南芥和几种叶菜类蔬菜的生长。
我们进一步证明，与 mock 接种的植物相比，在正常、盐和干旱胁迫条件下接种伯克霍尔德氏菌869T2后，拟南芥和小白菜植株的不同生长参数显著增加。转录组分析和定量实时PCR结果均表明，与盐处理的 mock 接种对照相比，盐胁迫下接种869T2菌株的拟南芥植株中，与植物激素信号转导途径相关的基因，包括生长素、赤霉素、细胞分裂素和脱落酸的表达水平发生了改变。此外，在盐和干旱胁迫下，接种869T2的拟南芥和小白菜植株中过氧化氢（HO）、电解质渗漏（EL）和丙二醛（MDA）的积累水平低于对照植株。
植物内生细菌菌株伯克霍尔德氏菌869T2可能影响各种植物激素反应，并减少氧化应激损伤，从而提高宿主植物对盐和干旱胁迫的耐受性。
