狼疮抗原La及RNA结合蛋白的鉴定与纯化。
Characterization and purification of lupus antigen La, and RNA-binding protein.
作者信息
Francoeur A M, Chan E K, Garrels J I, Mathews M B
出版信息
Mol Cell Biol. 1985 Mar;5(3):586-90. doi: 10.1128/mcb.5.3.586-590.1985.
Abstract
HeLa cell La antigen, an RNA-binding protein, was characterized by using two-dimensional gel electrophoresis. Eight isoelectric forms (pI 6 to 7) were observed, many containing phosphate. An in vitro translation product similar in size and antigenicity was identified. The HeLa cell protein purified by using an assay based on ribonucleoprotein reconstitution with adenovirus VA RNAI also comprised several isoelectric forms.
摘要
通过二维凝胶电泳对HeLa细胞中的La抗原（一种RNA结合蛋白）进行了表征。观察到8种等电形式（pI 6至7），许多含有磷酸盐。鉴定出一种在大小和抗原性上相似的体外翻译产物。使用基于腺病毒VA RNAI的核糖核蛋白重组分析纯化的HeLa细胞蛋白也包含几种等电形式。
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