Zakaria Norazua, Han Heping, Khalil Yaseen, Ashworth Michael, Yu Qin, Flower Ken C
Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Malaysia.
Australian Herbicide Resistance Initiative, School of Agriculture & Environment, University of Western Australia, Perth, Western Australia, Australia.
Pest Manag Sci. 2025 Jun;81(6):3196-3201. doi: 10.1002/ps.8690. Epub 2025 Feb 5.
The first case of glyphosate resistance was reported in a capeweed population from Western Australia in our previous study. This current study investigates the resistance mechanisms in the population.
Target-site EPSPS gene sequencing revealed two partial sequences of the EPSPS transcripts (1001 bp and 998 bp), and the full-length sequence (1551 bp) containing the 1001-bp transcript was cloned as it was found in the resistant plants. A known resistance-endowing target-site mutation in the 1551-bp transcript was identified in the resistant plants, resulting in the Pro-106-Ser substitution. The subpopulation derived from these mutant plants exhibited >10-fold resistance to glyphosate compared to the susceptible population. Additionally, the EPSPS gene (1551 bp) was constitutively expressed at a higher level (4.3-fold) in the resistant than in the susceptible populations. However, C-glyphosate foliar uptake was similar with no visual difference in C-glyphosate translocation from leaves to other parts of a plant, between the resistant and susceptible population.
Glyphosate resistance in the studied population is associated with both a target-site mutation (Pro-106-Ser) and increased EPSPS gene expression. © 2025 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
在我们之前的研究中，西澳大利亚的一个荠菜种群中首次报道了抗草甘膦的案例。本研究调查了该种群中的抗性机制。
靶位点EPSPS基因测序揭示了EPSPS转录本的两个部分序列（1001 bp和998 bp），并且在抗性植株中发现的包含1001 bp转录本的全长序列（1551 bp）被克隆。在抗性植株中鉴定出1551 bp转录本中一个已知的赋予抗性的靶位点突变，导致脯氨酸106被丝氨酸取代。与敏感种群相比，源自这些突变植株的亚种群对草甘膦表现出10倍以上的抗性。此外，EPSPS基因（1551 bp）在抗性种群中的组成型表达水平比敏感种群高（4.3倍）。然而，抗性和敏感种群之间，C-草甘膦的叶部吸收相似，且C-草甘膦从叶片向植物其他部位的转运没有明显差异。
所研究种群中的草甘膦抗性与靶位点突变（脯氨酸106被丝氨酸取代）和EPSPS基因表达增加有关。© 2025作者。由John Wiley & Sons Ltd代表化学工业协会出版的《害虫管理科学》。
