Sorge Sebastian, Girard Victor, Lampe Lena, Tixier Vanessa, Weaver Alexandra, Higgins Theresa, Gould Alex P
Laboratory of Physiology and Metabolism, The Francis Crick Institute, London NW1 1AT, UK.
Laboratory of Physiology and Metabolism, The Francis Crick Institute, London NW1 1AT, UK.
Dev Cell. 2025 Jun 9;60(11):1636-1648.e4. doi: 10.1016/j.devcel.2025.01.008. Epub 2025 Feb 4.
Diets composed of chemically pure components (holidic diets) are useful for determining the metabolic roles of individual nutrients. For the model organism Drosophila melanogaster, existing holidic diets are unable to support the rapid growth characteristic of the larval stage. Here, we use a nutrient co-optimization strategy across more than 50 diet variants to design HolFast, a holidic medium tailored to fast larval growth and development. We identify dietary amino acid ratios optimal for developmental speed but show that they compromise survival unless vitamins and sterols are co-optimized. Rapid development on HolFast is not improved by adding fatty acids, but it is dependent upon their de novo synthesis in the fat body via fatty acid synthase (FASN). HolFast outperforms other holidic diets, supporting rates of growth and development close to those of yeast-based diets and, under germ-free conditions, identical. HolFast has wide applications in nutritional and metabolic studies of Drosophila development.
由化学纯成分组成的饮食（全营养素饮食）对于确定单个营养素的代谢作用很有用。对于模式生物黑腹果蝇而言，现有的全营养素饮食无法支持幼虫阶段特有的快速生长。在此，我们通过对50多种饮食变体采用营养共同优化策略，设计出了HolFast，这是一种专为幼虫快速生长和发育量身定制的全营养素培养基。我们确定了对发育速度最佳的饮食氨基酸比例，但表明除非维生素和固醇共同优化，否则这些比例会影响存活率。在HolFast上的快速发育不会因添加脂肪酸而得到改善，但它依赖于脂肪酸合酶（FASN）在脂肪体中从头合成脂肪酸。HolFast优于其他全营养素饮食，支持的生长和发育速度接近基于酵母的饮食，并且在无菌条件下，二者相同。HolFast在果蝇发育的营养和代谢研究中有广泛应用。
