Huang Tao, Shao Bo, Gong Zhong-Cheng
Department of Oral and Maxillofacial Surgery, Affiliated Stomatology Hospital, Wenzhou Medical University, Wenzhou City, No. 1288 Longyao Avenue, Wenzhou City, Zhejiang Province PR China.
Oncological Department of Oral and Maxillofacial Surgery, The Affiliated Stomatology Hospital of the First Affiliated Hospital of Xinjiang Medical University, No. 137, Li Yu Shan South Road, Urumqi 830054, Xinjiang, PR China.
J Stomatol Oral Maxillofac Surg. 2025 Jun;126(3S):102270. doi: 10.1016/j.jormas.2025.102270. Epub 2025 Feb 3.
This study investigates the effect of miR-141 overexpression on the proliferation and apoptosis of tongue squamous cell carcinoma (TSCC) cell lines (Tscca and Tca8113). TSCC cells were stably transfected with a miR-141 lentivirus (Ubi-miR-141-SV40-EGFP-IRES-puromycin, miR-141 group) or an empty vector (Ubi-MCS-SV40-EGFP-IRES-puromycin, miR-NC group), with untreated cells serving as the Blank group. miR-141 expression was evaluated by rt-qPCR, transfection efficiency and apoptosis were assessed by flow cytometry, and cell proliferation was measured using the CCK-8 assay. Western blot and rt-qPCR were used to analyze the protein and mRNA expression of phosphatase and tensin homolog (PTEN) and B-cell lymphoma-2 (BCL2). Results: Flow cytometry confirmed successful establishment of stably transfected cell lines. The CCK-8 assay showed no significant difference in proliferation between the miR-NC and Blank groups (P > 0.05), while proliferation was significantly reduced in the miR-141 group. Apoptosis was significantly higher in the miR-141 group compared to both the miR-NC and Blank groups. Western blot and rt-qPCR analyses revealed upregulation of pten gene and PTEN protein expression, as well as downregulation of bcl2 gene and BCL2 protein expression in the miR-141 group relative to the miR-NC and Blank groups. Conclusion: In TSCC cells with high miR-141 expression, pten gene and PTEN protein levels were elevated, while bcl2 gene and BCL2 protein levels were reduced. These findings indicate that miR-141 may regulate the expression of PTEN and BCL2 at both the gene and protein levels, thereby influencing cell proliferation, migration, and apoptosis. To our knowledge, this is the first study to explore the effects of miR-141 transfection in TSCC, providing valuable insights into its role in tumor progression and potential regulatory targets.
本研究调查了miR-141过表达对舌鳞状细胞癌（TSCC）细胞系（Tscca和Tca8113）增殖和凋亡的影响。TSCC细胞用miR-141慢病毒（泛素-miR-141-SV40-EGFP-IRES-嘌呤霉素，miR-141组）或空载体（泛素-MCS-SV40-EGFP-IRES-嘌呤霉素，miR-NC组）进行稳定转染，未处理的细胞作为空白组。通过rt-qPCR评估miR-141表达，通过流式细胞术评估转染效率和凋亡，使用CCK-8法测量细胞增殖。采用蛋白质免疫印迹法和rt-qPCR分析磷酸酶和张力蛋白同源物（PTEN）及B细胞淋巴瘤-2（BCL2）的蛋白质和mRNA表达。结果：流式细胞术证实成功建立稳定转染细胞系。CCK-8法显示miR-NC组和空白组之间增殖无显著差异（P>0.05），而miR-141组增殖显著降低。与miR-NC组和空白组相比，miR-141组凋亡显著更高。蛋白质免疫印迹法和rt-qPCR分析显示，相对于miR-NC组和空白组，miR-141组中pten基因和PTEN蛋白表达上调，bcl2基因和BCL2蛋白表达下调。结论：在miR-141高表达的TSCC细胞中，pten基因和PTEN蛋白水平升高，而bcl2基因和BCL2蛋白水平降低。这些发现表明，miR-141可能在基因和蛋白质水平上调节PTEN和BCL2的表达，从而影响细胞增殖、迁移和凋亡。据我们所知，这是第一项探索miR-141转染对TSCC影响的研究，为其在肿瘤进展中的作用和潜在调控靶点提供了有价值的见解。
