Kim Hyeonju, Ahn Meejung, Jung Kyungsook, Hong Sungmoo, Ko Deokho, Kang Taeyoung, Shin Taekyun, Kim Jeongtae
Department of Veterinary Medicine, Graduate School, Jeju National University, Jeju, Republic of Korea.
Department of Animal Science, College of Life Science, Sangji University, Wonju, Republic of Korea.
J Vet Med Sci. 2025 Mar 10;87(3):301-307. doi: 10.1292/jvms.24-0231. Epub 2025 Feb 4.
Lipocalin-2, a siderophore-binding protein, is an antimicrobial, exerting both pro-inflammatory and anti-inflammatory actions. Lipocalin-2 is also involved in glial activation, matrix metalloproteinase stabilization, and cellular iron flux, all of which play roles in autoimmune diseases. The present study aimed to determine the expression of lipocalin-2 in the eyes of Lewis rats with interphotoreceptor-binding protein-induced experimental autoimmune uveitis (EAU). Significantly elevated serum lipocalin-2 levels were also detected in rats by enzyme-linked immunosorbent assay (ELISA). Lipocalin-2 immunostaining was detected predominantly in activated glial cells, including glutamine synthase-positive Müller cells, and ionized calcium-binding adaptor molecule 1-positive microglia and macrophages in EAU rats. Taken together, the results presented herein show that lipocalin-2 is a potential diagnostic marker for uveitis.
lipocalin-2是一种与铁载体结合的蛋白质，具有抗菌作用，兼具促炎和抗炎活性。lipocalin-2还参与神经胶质细胞活化、基质金属蛋白酶稳定以及细胞铁流量调节，所有这些在自身免疫性疾病中都发挥作用。本研究旨在确定在光感受器间结合蛋白诱导的实验性自身免疫性葡萄膜炎（EAU）Lewis大鼠眼中lipocalin-2的表达情况。通过酶联免疫吸附测定（ELISA）还检测到大鼠血清中lipocalin-2水平显著升高。在EAU大鼠中，lipocalin-2免疫染色主要在活化的神经胶质细胞中检测到，包括谷氨酰胺合成酶阳性的Müller细胞以及离子钙结合衔接分子1阳性的小胶质细胞和巨噬细胞。综上所述，本文呈现的结果表明lipocalin-2是葡萄膜炎的潜在诊断标志物。
