Prasath N, Arul Senghor K A, Vinodhini V M, Anuradha M
SRM Medical College Hospital and Research Centre, SRM Institute of Science and Technology, Kattankulathur, Chennai, Tamil Nadu, 603 203, India.
Reprod Sci. 2025 Mar;32(3):748-756. doi: 10.1007/s43032-025-01803-z. Epub 2025 Feb 5.
This research delves into the association of Kisspeptin and the KISS gene (rs35431622) single nucleotide polymorphism with circulating sex hormones and semen parameters in males diagnosed with infertility. Eighty male participants were recruited from fertility clinic, were divided into two groups: Group A, characterized by normal sperm count and Group B, exhibiting low count. The analysis involved assessing hormone levels Kisspeptin, follicle stimulating hormone, luteinizing hormone, total testosterone and sex hormone binding globulin by utilizing ELISA, investigating the association of the KISS gene SNP with these variables through Tetra amplification refractory mutation system-PCR and Gel Electrophoresis. The findings revealed that males a low sperm count displayed notably reduced levels of Kisspeptin and free testosterone, accompanied by increased luteinizing hormone and follicle stimulating hormone. The KISS gene SNP rs35431622 (Q36R) demonstrated a 53.3% frequency of the heterozygous mutant TA genotype and OR 0.19 (CI 0.05-0.71) among infertile males, indicating its potential implications a risk factor for male infertility. This study emphasizes the significance of Kisspeptin in overseeing male reproductive health and highlights its potential as a marker for hormonal dysregulation in male infertility.
本研究深入探讨了亲吻素（Kisspeptin）与KISS基因（rs35431622）单核苷酸多态性与诊断为不育症男性的循环性激素及精液参数之间的关联。从生育诊所招募了80名男性参与者，分为两组：A组精子计数正常，B组精子计数低。分析包括通过酶联免疫吸附测定法（ELISA）评估亲吻素、促卵泡生成素、促黄体生成素、总睾酮和性激素结合球蛋白的激素水平，通过四重扩增阻滞突变系统聚合酶链反应（Tetra amplification refractory mutation system-PCR）和凝胶电泳研究KISS基因单核苷酸多态性与这些变量之间的关联。研究结果显示，精子计数低的男性亲吻素和游离睾酮水平显著降低，同时促黄体生成素和促卵泡生成素增加。KISS基因单核苷酸多态性rs35431622（Q36R）在不育男性中杂合突变TA基因型的频率为53.3%，比值比（OR）为0.19（置信区间CI为0.05 - 0.71），表明其作为男性不育风险因素的潜在影响。本研究强调了亲吻素在监测男性生殖健康中的重要性，并突出了其作为男性不育激素失调标志物的潜力。
