Emre Akın Süleyman, Mehtap Savran, Cem Doğan, İlter İlhan, Melih Arlıoğlu, Özlem Özmen, Serdar Sezer, Ekrem Çamaş Hasan, Rasih Yazkan
Department of Thoracic Surgery, Faculty of Medicine, Suleyman Demirel University, Isparta, Turkey.
Department of Medical Pharmacology, Faculty of Medicine, Suleyman Demirel University, Isparta, Turkey.
Eur J Trauma Emerg Surg. 2025 Feb 7;51(1):95. doi: 10.1007/s00068-025-02767-0.
This study aimed to investigate the hypothesis that cannabidiol (CBD), with known anti-inflammatory and anti-apoptotic effects, would reduce the severity of acute lung injury in pulmonary contusion following blunt chest trauma.
Forty male Wistar Albino rats were randomly divided into four groups, each consisting of 10 rats: Sham, Trauma, Trauma + CBD, and CBD. The rats were treated with a single dose of 5 mg/kg CBD intraperitoneally 30 min before trauma. Then, the trauma were exposed to a weight of 200 g and a height of 1 m. After sacrifice, the lung tissues were removed for histopathological, immunohistochemical, biochemical, and genetic analyses.
Pulmonary injury of trauma group led to increases in tumor necrosis factor α, caspase-3, caspase-9, Bcl-2-associated X protein expressions, total oxidant status, oxidative stress index levels, and decreases in B-cell lymphoma expression and total antioxidant levels. Additionally, inflammatory cell infiltration, damage-related emphysema, pronounced hyperemia, and increased septal tissue thickness were observed histopathologically. CBD treatment ameliorated all these findings.
CBD reduces lung damage in lung contusions caused by blunt chest trauma through its anti-inflammatory and antiapoptotic effects. More detailed studies investigating other important intracellular pathways are needed.
本研究旨在探讨假说，即已知具有抗炎和抗凋亡作用的大麻二酚（CBD）可减轻钝性胸部创伤后肺挫伤中急性肺损伤的严重程度。
40只雄性Wistar白化大鼠随机分为四组，每组10只：假手术组、创伤组、创伤+CBD组和CBD组。在创伤前30分钟，给大鼠腹腔注射单剂量5mg/kg的CBD。然后，使创伤组大鼠承受200g的重量和1m的高度。处死后，取出肺组织进行组织病理学、免疫组织化学、生化和基因分析。
创伤组的肺损伤导致肿瘤坏死因子α、半胱天冬酶-3、半胱天冬酶-9、Bcl-2相关X蛋白表达、总氧化剂状态、氧化应激指数水平升高，B细胞淋巴瘤表达和总抗氧化剂水平降低。此外，组织病理学观察到炎性细胞浸润、损伤相关性肺气肿、明显充血和间隔组织厚度增加。CBD治疗改善了所有这些结果。
CBD通过其抗炎和抗凋亡作用减轻钝性胸部创伤所致肺挫伤中的肺损伤。需要进行更详细的研究来探究其他重要的细胞内途径。
