Steele T H, Gottstein J H, Challoner-Hue L
Ren Physiol. 1985;8(2):65-72. doi: 10.1159/000173037.
We treated 20-week-old spontaneously hypertensive rats (SHR) with either placebo or hydralazine, reserpine and hydrochlorothiazide for 1 month. Mean arterial pressure in treated SHR averaged 113 +/- 7 mm Hg (mean +/- SE), compared to 162 +/- 12 mm Hg in animals receiving placebo (p less than 0.01). Glomerular filtration rate (GFR) and sodium excretion were similar in both groups. In isolated perfused kidneys, the GFR and sodium excretion were significantly greater in the treatment group than in the placebo group at a perfusion pressure of 140 mm Hg (p less than 0.01). Renal vascular resistance (RVR) of kidney from treated SHR was no different from RVR of kidney from placebo SHR. Hydralazine (6 mM) and diazoxide (4 mM) increased the GFR and sodium excretion of isolated SHR kidney perfused at 140 mm Hg (p less than 0.05), but decreased RVR significantly (p less than 0.05). We conclude that prolonged antihypertensive treatment renders higher GFR values to isolated SHR kidneys perfused at 140 mm Hg, with sodium excretion varying in proportion to the GFR. The addition of vasodilators to the perfusate of isolated SHR kidneys partially reproduced these changes, but only at extremely high concentrations unlike to be attained in vivo.
我们用安慰剂或肼屈嗪、利血平和氢氯噻嗪对20周龄的自发性高血压大鼠（SHR）进行了为期1个月的治疗。治疗后的SHR平均动脉压为113±7 mmHg（平均值±标准误），而接受安慰剂的动物平均动脉压为162±12 mmHg（p<0.01）。两组的肾小球滤过率（GFR）和钠排泄相似。在离体灌注肾脏中，在140 mmHg的灌注压力下，治疗组的GFR和钠排泄显著高于安慰剂组（p<0.01）。治疗后的SHR肾脏的肾血管阻力（RVR）与接受安慰剂的SHR肾脏的RVR没有差异。肼屈嗪（6 mM）和二氮嗪（4 mM）增加了在140 mmHg灌注的离体SHR肾脏的GFR和钠排泄（p<0.05），但显著降低了RVR（p<0.05）。我们得出结论，长期抗高血压治疗使在140 mmHg灌注的离体SHR肾脏具有更高的GFR值，钠排泄与GFR成比例变化。向离体SHR肾脏的灌注液中添加血管扩张剂部分重现了这些变化，但仅在极高浓度下，与体内可能达到的浓度不同。
