Hao Ting, Cui Lijuan, Wang Jinzhi, Lei Yinru, Li Wei, Wang Rumiao, Wang Shaokun, Li Jing, Zhai Xiajie, Zhang Manyin, Zhao Xinsheng
State Key Laboratory of Wetland Conservation and Restoration, Chinese Academy of Forestry, Beijing, 100091, China; Institute of Ecological Conservation and Restoration, Chinese Academy of Forestry, Beijing, 100091, China; Beijing Key Laboratory of Wetland Ecological Function and Restoration, Beijing, 100091, China; Beijing Hanshiqiao National Wetland Ecosystem Research Station, Beijing, 101399, China.
State Key Laboratory of Wetland Conservation and Restoration, Chinese Academy of Forestry, Beijing, 100091, China; Institute of Ecological Conservation and Restoration, Chinese Academy of Forestry, Beijing, 100091, China; Beijing Key Laboratory of Wetland Ecological Function and Restoration, Beijing, 100091, China; Beijing Hanshiqiao National Wetland Ecosystem Research Station, Beijing, 101399, China.
J Environ Manage. 2025 Mar;376:124243. doi: 10.1016/j.jenvman.2025.124243. Epub 2025 Feb 8.
In constructed wetlands (CWs), the rhizosphere acts as a critical zone for phosphorus (P) mineralization and the sustenance of microbial activity. However, low temperatures can significantly inhibit microbial processes and enzyme activities, particularly phosphatase activities that are crucial for P mineralization. Despite this, the impact of low temperatures on phosphorus-related phosphatase activities and the composition and function of microbial communities during the winter is not fully comprehended. This study investigates the response of phosphatase activity and P-mineralizing microbial communities to decreased winter temperatures in two contrasting CWs. The research was conducted at two CWs, designated as LCW and HCW, to assess the effects of winter temperatures on phosphatase activity and P-mineralizing microbial communities, with a focus on the suppressive effects of low temperatures. The study suggests that while the acid phosphatase (ACP) in HCW remains stable, the LCW shows a significant decrease. Correspondingly, the phoC community in HCW shows minimal variation, whereas community in LCW exhibits significant variation. Alkaline phosphatase (ALP) exhibits greater tolerance to low temperatures compared to ACP. The random forest analysis reveals that Pseudomonas is the primary contributor to ALP secretion, whereas Stenotrophomonas, possessing the phoC gene, is identified as the key contributor to ACP secretion during the winter season. ALP activity was significantly affected by soil temperature and soluble sugar, whereas ACP activity was strongly negatively associated with pH, organic acid and total organic carbon (TOC). Additionally, low temperatures caused phoC-harboring bacteria to cluster more closely, which may help maintain their functional potential in P mineralization. These findings highlight the significance of regulating ice thickness in CWs to optimize P availability, especially during the colder seasons, by revealing the microbial community's adaptive mechanisms to environmental stress. This research elucidates the complex relationship between winter temperature gradients and phosphorus mineralization processes. The findings are particularly relevant for optimizing nutrient management in wetland ecosystems under temperature fluctuations and contribute significantly to the discourse on sustainable management and the maintenance of microbial mineralization processes in the context of climate change.
在人工湿地（CWs）中，根际是磷（P）矿化和微生物活性维持的关键区域。然而，低温会显著抑制微生物过程和酶活性，尤其是对P矿化至关重要的磷酸酶活性。尽管如此，冬季低温对与磷相关的磷酸酶活性以及微生物群落组成和功能的影响尚未完全了解。本研究调查了两种不同人工湿地中磷酸酶活性和P矿化微生物群落对冬季温度降低的响应。研究在两个分别命名为LCW和HCW的人工湿地上进行，以评估冬季温度对磷酸酶活性和P矿化微生物群落的影响，重点关注低温的抑制作用。研究表明，虽然HCW中的酸性磷酸酶（ACP）保持稳定，但LCW中的ACP显著下降。相应地，HCW中的phoC群落变化最小，而LCW中的群落则表现出显著变化。碱性磷酸酶（ALP）比ACP对低温具有更高的耐受性。随机森林分析表明，假单胞菌是ALP分泌的主要贡献者，而拥有phoC基因的嗜麦芽窄食单胞菌被确定为冬季ACP分泌的关键贡献者。ALP活性受土壤温度和可溶性糖的显著影响，而ACP活性与pH、有机酸和总有机碳（TOC）呈强烈负相关。此外，低温导致携带phoC的细菌聚集更紧密，这可能有助于维持它们在P矿化中的功能潜力。这些发现通过揭示微生物群落对环境压力的适应机制，突出了调节人工湿地冰厚度以优化P有效性的重要性，特别是在较冷季节。本研究阐明了冬季温度梯度与磷矿化过程之间的复杂关系。这些发现对于在温度波动下优化湿地生态系统中的养分管理尤为相关，并为气候变化背景下可持续管理和微生物矿化过程维持的讨论做出了重要贡献。
