Jiang Jia, Hu Bingyang, Gao Xuheng, Cui Yongyuan, Qian Le, Xu Jianqiang, Liu Shengming
College of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang 471023, China.
Henan Province Engineering Technology Research Center of Green Plant Protection, Luoyang 471023, China.
J Agric Food Chem. 2025 Feb 19;73(7):4001-4011. doi: 10.1021/acs.jafc.4c13301. Epub 2025 Feb 9.
Fusarium head blight (FHB), caused by , is a significant disease of wheat. In this study, the sensitivity of 102 isolates to fluopyram was determined based on inhibition of conidia germination and mycelial growth. The EC value ranged from 0.0952 to 0.2717 μg/mL and1.0326 to 4.8512 μg/mL, with the mean EC value of 0.1631 ± 0.0383 and 2.5638 ± 0.7500 μg/mL, respectively. The resistance of the fluopyram-resistant mutants could be stably inherited, and the survival fitness of the mutants was higher than that of the parental isolates. Positive cross-resistance was observed between fluopyram and pydiflumetofen, but no cross-resistance was observed between fluopyram and epoxiconazole, phenamacril, metconazole, carbendazim, or fludioxonil. Amino acid substitution A73 V was detected on the FgSDHC subunit, and the gene was significantly upregulated after exposure to fluopyram. These results indicate that fluopyram exerts its inhibitory activity by targeting the gene of .
由镰刀菌引起的小麦赤霉病是小麦的一种重要病害。在本研究中，基于对分生孢子萌发和菌丝生长的抑制作用，测定了102株镰刀菌分离株对氟吡菌酰胺的敏感性。其EC值范围分别为0.0952至0.2717μg/mL和1.0326至4.8512μg/mL，平均EC值分别为0.1631±0.0383和2.5638±0.7500μg/mL。氟吡菌酰胺抗性突变体的抗性能够稳定遗传，且突变体的生存适合度高于亲本分离株。氟吡菌酰胺与氟唑菌酰羟胺之间存在正向交互抗性，但氟吡菌酰胺与环氧唑、苯醚甲环唑、丙环唑、多菌灵或咯菌腈之间未观察到交互抗性。在FgSDHC亚基上检测到氨基酸取代A73V，且在接触氟吡菌酰胺后该基因显著上调。这些结果表明，氟吡菌酰胺通过靶向镰刀菌的基因发挥其抑制活性。
