Discovery of novel vaccine candidates based on the immunogenic epitopes derived from membrane proteins.

PURPOSE

Due to the widespread distribution and importance of infection as a parasitic zoonosis, multi-epitope vaccine design was implemented using a set of immunodominant epitopes screened out of a wide scope of membrane proteins.

MATERIALS AND METHODS

On this basis, 5 vaccine candidates were created using linkers ([GGGGS], KK, AAY, GPGPG, GDGDG, EAAAK) and adjuvants (RS-09 peptide, resuscitation-promoting factor E [RpfE] and 50S ribosomal protein, human interferon [IFN]-γ).

RESULTS

Polytopes with RS-09 alone (Toxo-App) and with IFN-γ (Toxo-Apfn), and one with 50S ribosomal protein (Toxo-Ribos) showed the highest immunogenicity during prediction, and their 3-dimensional structure was refined. Protein-protein docking and molecular dynamics simulation analysis was done between the Toxo-App and human toll-like receptor (TLR)-4, rendering a stable connection. Codon optimization and cloning was done ultimately for the selected vaccine candidate.

CONCLUSION

In conclusion, potent multi-epitope vaccine candidates were designed against toxoplasmosis using a diverse set of techniques, while further wet experiments are recommended.