Peng Bo, Zhu Xinyi, Geng Li, Xu Wenping, Cheng Jiagao, Tao Liming, Zhang Yang
Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai, 200237, China.
Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai, 200237, China.
Food Chem Toxicol. 2025 Apr;198:115316. doi: 10.1016/j.fct.2025.115316. Epub 2025 Feb 8.
This study evaluates the developmental and cardiotoxic effects of cyhalofop-butyl, a commonly used herbicide in rice agriculture, on zebrafish (Danio rerio) embryos. Despite its widespread application, the risk assessment of cyhalofop-butyl for aquatic organisms, especially fish, is still lacking. Focusing on the cardiac system, we used a zebrafish model to evaluate developmental abnormalities, changes in cardiac morphology and function, markers of oxidative stress, and altered gene expression. The results suggest that cyhalofop-butyl induces oxidative stress, lipid accumulation, and apoptosis in zebrafish embryos. In addition, it can lead to abnormal embryonic development and cardiac morphological dysfunction (such as pericardial edema, decreased heart rate, and red blood cell (RBC) flow rate, and cardiac linearization). Cyhalofop-butyl also significantly alters the expression of cardiac-related genes, including myl7, vmhc, myh6, nkx2.5, tbx5, nppa, has2, and myh7. In summary, cyhalofop-butyl elicits both dysplasia and cardiotoxicity in zebrafish embryos, highlighting the need for further safety risk evaluation of this herbicide in aquatic ecosystems.
本研究评估了在水稻种植中常用的除草剂氰氟草酯对斑马鱼（Danio rerio）胚胎的发育和心脏毒性作用。尽管氰氟草酯已广泛应用，但其对水生生物尤其是鱼类的风险评估仍较为缺乏。以心脏系统为重点，我们使用斑马鱼模型评估发育异常、心脏形态和功能变化、氧化应激标志物以及基因表达改变。结果表明，氰氟草酯可诱导斑马鱼胚胎发生氧化应激、脂质积累和细胞凋亡。此外，它可导致胚胎发育异常和心脏形态功能障碍（如心包水肿、心率降低、红细胞（RBC）流速以及心脏直线化）。氰氟草酯还显著改变心脏相关基因的表达，包括myl7、vmhc、myh6、nkx2.5、tbx5、nppa、has2和myh7。总之，氰氟草酯在斑马鱼胚胎中引发发育异常和心脏毒性，凸显了对该除草剂在水生生态系统中进行进一步安全风险评估的必要性。
