Milanov M L, Shikalanov S, Ianakieva S, Iolov I I
Vet Med Nauki. 1985;22(3):9-14.
Colorimetric analysis, biochemical methods, and the complement-fixation test were employed to study 3 Australian and 5 Bulgarian strains of Brucella ovis. Determined were some biochemical manifestations, the adaptation capacity with regard to aerobic conditions of cultivation without serum, the amount of polysaccharides contained in the strains, and the properties of the antigens obtained as checked via CFT. Milanov's method was employed for antigen production. Three of the Bulgarian strains showed definite advantages in the commercial production of antigens for the serologic diagnosis of the Brucella ovis infection in sheep.