Snyder E L, Ferri P, Brown R, Gallup P, Roberts S
Vox Sang. 1985;48(5):269-75. doi: 10.1111/j.1423-0410.1985.tb00182.x.
We studied the storage characteristics of platelet concentrates prepared in polyolefin (PL-732) and thin-film polyvinyl chloride (CLX) plastic bags and stored on a newly designed flatbed reciprocal motion agitator with a 1.5 inch (3.8 cm) lateral movement and an oscillation frequency of 70 cycles/min. We also studied the ability of this device to resuspend the platelet button formed after preparative centrifugation. Results showed that platelet concentrates stored on the 1.5-inch shaker had storage characteristics equivalent to those reported for the conventional 70 cycles/min, 1-inch (2.5-cm) lateral movement flatbed shaker. Due to a more rapid acceleration and deceleration rate, however, the 1.5-inch shaker resuspended the platelet button formed after preparative centrifugation much more efficiently than did the less dynamic 1-inch shaker. Furthermore, with either agitator, platelet aggregates in the CLX bag were more readily resuspended than were aggregates in the PL-732 bag due in part to differences in plastic bag wall elasticity.
我们研究了在聚烯烃（PL - 732）和薄膜聚氯乙烯（CLX）塑料袋中制备的血小板浓缩物的储存特性，并将其储存在新设计的平板往复式搅拌器上，该搅拌器具有1.5英寸（3.8厘米）的横向移动和70次/分钟的振荡频率。我们还研究了该装置重新悬浮制备性离心后形成的血小板凝块的能力。结果表明，储存在1.5英寸振荡器上的血小板浓缩物的储存特性与传统的70次/分钟、1英寸（2.5厘米）横向移动平板振荡器所报道的特性相当。然而，由于加速和减速速度更快，1.5英寸振荡器比动态较小的1英寸振荡器更有效地重新悬浮制备性离心后形成的血小板凝块。此外，使用任一搅拌器时，CLX袋中的血小板聚集体比PL - 732袋中的聚集体更容易重新悬浮，部分原因是塑料袋壁弹性的差异。
