Mazzone R W, Durand C M, West J B
J Microsc. 1979 Nov;117(2):269-84. doi: 10.1111/j.1365-2818.1979.tb01183.x.
Analysis of pulmonary structure-function relationships by microscopy requires that the lung be fixed under carefully controlled physiological conditions, since lung structure is extremely responsive to the relationship between airway and vascular pressures. Unfortunately, standard post-mortem fixation techniques leave some doubt as to the exact relationship between these pressures during fixation. This problem can be circumvented by stabilizing lung structure by rapid freezing under carefully controlled physiologic conditions. Using ethylene glycol in a freeze substitution technique we have developed procedures which yield a degree of preservation compatible with the high degree of resolution of the electron microscope. These can be used to obtain a more detailed understanding of pulmonary structure-function relationships under well-defined physiological conditions.
通过显微镜分析肺的结构与功能关系，要求肺在严格控制的生理条件下进行固定，因为肺结构对气道和血管压力之间的关系极为敏感。不幸的是，标准的尸检固定技术使得在固定过程中这些压力的确切关系存在一些疑问。通过在严格控制的生理条件下快速冷冻来稳定肺结构，可以规避这个问题。我们利用乙二醇的冷冻置换技术开发了一些程序，这些程序能够实现与电子显微镜的高分辨率相匹配的保存程度。这些程序可用于在明确的生理条件下更详细地了解肺的结构与功能关系。
