Zhu Bo, Wang Tianzhen, Niu Qunhao, Wang Zezhao, Hay El Hamidi, Xu Lei, Chen Yan, Zhang Lupei, Gao Xue, Gao Huijiang, Cao Yang, Zhao Yumin, Xu Lingyang, Li Junya
State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing, China.
Northern Agriculture and Livestock Husbandry Technology Innovation Center, Hohhot, China.
Commun Biol. 2025 Feb 10;8(1):208. doi: 10.1038/s42003-025-07604-z.
Fatty acid composition (FA) is an important indicator of meat quality in beef cattle. We investigated potential functional candidate genes for FA in beef cattle by integrating genomic and transcriptomic dataset through multiple strategies. In this study, we observed 65 SNPs overlapping with five candidate genes (CCDC57, FASN, HDAC11, ALG14, and ZMAT4) using two steps association based on the imputed sequencing variants. Using multiple traits GWAS, we further identified three significant SNPs located in the upstream of FASN and one SNP (chr19:50779529) was embedded in FASN. Of those, two SNPs were further identified as the cis-eQTL based on transcriptomic analysis of muscle tissues. Moreover, the knockdown of FASN yielded a significant reduction in intracellular triglyceride content in preadipocytes and impeded lipid droplet accumulation in adipocytes. RNA-seq analysis of preadipocytes with FASN interference revealed that the differentially expressed genes were enriched in cell differentiation and lipid metabolic pathway. Our study underscored the indispensable role of FASN in orchestrating adipocyte differentiation, and fatty acid metabolism. The integrative analysis with multiple strategies may contribute to the understanding of the genetic architecture of FA in farm animals.
脂肪酸组成（FA）是肉牛肉质的一个重要指标。我们通过多种策略整合基因组和转录组数据集，研究了肉牛FA的潜在功能候选基因。在本研究中，我们基于推算的测序变异，通过两步关联分析观察到65个单核苷酸多态性（SNP）与五个候选基因（CCDC57、FASN、HDAC11、ALG14和ZMAT4）重叠。使用多性状全基因组关联研究（GWAS），我们进一步鉴定出位于FASN上游的三个显著SNP，并且一个SNP（chr19:50779529）位于FASN内部。其中，基于肌肉组织的转录组分析，有两个SNP被进一步鉴定为顺式表达数量性状位点（cis-eQTL）。此外，FASN的敲低导致前脂肪细胞内甘油三酯含量显著降低，并阻碍脂肪细胞中脂滴的积累。对FASN干扰的前脂肪细胞进行RNA测序分析表明，差异表达基因在细胞分化和脂质代谢途径中富集。我们的研究强调了FASN在协调脂肪细胞分化和脂肪酸代谢中不可或缺的作用。采用多种策略进行综合分析可能有助于理解家畜中FA的遗传结构。
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