Yu Jia, Naseem Sajida, Park Sungkwon, Hur Sunjin, Choi Yoonbin, Lee Teahyung, Li Xiangzi, Choi Seongho
Department of Animal Science, Chungbuk National University, Cheongju 28644, Republic of Korea.
Engineering Research Center of North-East Cold Region Beef Cattle Science & Technology Innovation Ministry of Education, Department of Animal Science, Yanbian University, Yanji 133002, China.
Animals (Basel). 2025 Mar 20;15(6):897. doi: 10.3390/ani15060897.
Genetic polymorphisms have a great impact on enhancing quantitative traits in cattle. In this study, Fatty acid synthase () g. 16024 (A>G), Stearoyl-CoA desaturase () g. 10329 (C>T), and pleomorphic adenoma gene () g. 25003338 (C>G) genotypic and allelic polymorphisms were evaluated, along with their associations with fatty acid composition, adipogenic gene expression, and carcass characteristics (carcass weight, yield grade, backfat thickness, and marbling score) in Hanwoo steers. A total of 128 Hanwoo steers were selected for this study and the Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to identify polymorphism of these genes. The AG genotype and G allele in g. 16024 (A>G), CT genotype and T allele in g. 10329 (C>T), and GG genotype and G allele in g. 25003338 (C>G) showed higher frequency and positively correlated with carcass traits, yield, and quality grades. Fatty acid composition results indicate that C18:3n-6, C20:1, and C20:2n-6 were significantly higher in the AA genotype of gene, C14:1 and C18:3n-6 in the CC genotype, and C16:1 in the TT genotype of gene. C12:0, C14:0, C16:1, C18:0, and C20:0 were higher in the CC genotype of gene. Furthermore, RT-qPCR analysis of adipogenesis-related genes (AMP-activated protein kinase-α (), Carnitine palmitoyl transferase-1β (), G-coupled protein receptor-43 (), and ) across different SNP genotypes suggests a systemic interaction between genetic factors and adipogenesis in beef cattle. This study emphasizes the significance of g. 16024 (A>G), g. 10329 (C>T), and g. 25003338 (C>G) SNPs for genetic selection to enhance beef quality and elucidate lipid metabolic pathways in Hanwoo cattle.
基因多态性对提高牛的数量性状有很大影响。在本研究中，评估了脂肪酸合酶（FAS）g.16024（A>G）、硬脂酰辅酶A去饱和酶（SCD）g.10329（C>T）和多形性腺瘤基因（PLAG1）g.25003338（C>G）的基因型和等位基因多态性，以及它们与韩牛阉牛的脂肪酸组成、脂肪生成基因表达和胴体特性（胴体重、产量等级、背膘厚度和大理石花纹评分）之间的关联。本研究共选择了128头韩牛阉牛，并采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法来鉴定这些基因的多态性。FAS基因g.16024（A>G）中的AG基因型和G等位基因、SCD基因g.10329（C>T）中的CT基因型和T等位基因以及PLAG1基因g.25003338（C>G）中的GG基因型和G等位基因频率较高，且与胴体性状、产量和品质等级呈正相关。脂肪酸组成结果表明，FAS基因的AA基因型中C18:3n-6、C20:1和C20:2n-6显著更高，SCD基因的CC基因型中C14:1和C18:3n-6更高，PLAG1基因的TT基因型中C16:1更高。PLAG1基因的CC基因型中C12:0、C14:0、C16:1、C18:0和C20:0更高。此外，对不同SNP基因型的脂肪生成相关基因（AMP激活的蛋白激酶-α（AMPK-α）、肉碱棕榈酰转移酶-1β（CPT-1β）、G蛋白偶联受体-43（GPR43）和PPARγ）进行RT-qPCR分析，表明肉牛中遗传因素与脂肪生成之间存在系统性相互作用。本研究强调了FAS基因g.16024（A>G）、SCD基因g.10329（C>T）和PLAG1基因g.25003338（C>G）的单核苷酸多态性在遗传选择以提高牛肉品质和阐明韩牛脂质代谢途径方面的重要性。
