Guoth J, Varga B
Acta Physiol Hung. 1985;65(1):53-5.
Proestrous rats were treated with porcine follicular fluid (pFF) or porcine serum (pS) extract, afterwards they were put with males together. Next morning, the sperm positive females were considered as day 1 pregnant animals. On days 2, 8 and 14 of pregnancy serum progesterone level was determined by RIA. On days 2 and 8 of pregnancy serum progesterone level of pFF treated animals was significantly lower than that of pS treated ones, but it was not different from the controls on day 14 of pregnancy. The decreased progesterone level indicates that there are biologically active endogenous substances in the pFF (presumably inhibin or granulosa cell luteinization inhibitor) which may responsible for some forms of corpus luteum insufficiency and for some unexplained infertility cases.
对处于动情前期的大鼠用猪卵泡液（pFF）或猪血清（pS）提取物进行处理，之后将它们与雄性大鼠放在一起。次日清晨，精子呈阳性的雌性大鼠被视为妊娠第1天的动物。在妊娠第2、8和14天，通过放射免疫分析法测定血清孕酮水平。在妊娠第2天和第8天，用pFF处理的动物血清孕酮水平显著低于用pS处理的动物，但在妊娠第14天与对照组无差异。孕酮水平降低表明pFF中存在生物活性内源性物质（可能是抑制素或颗粒细胞黄体化抑制剂），这可能是某些形式的黄体功能不全及一些不明原因不孕症的原因。
