Mercury exposure poses significant threats to human health, particularly in its organic form, methylmercury (MeHg). Diet is the main pathway for human MeHg exposure, especially through seafood consumption. In this context, numerous studies have established seafood MeHg concentration datasets to assess MeHg-related health risks from seafood consumption. However, existing datasets are limited to specific regions and short-term observations, making it difficult to support continuous and dynamic assessments of global MeHg-related health risks. This study takes a bottom-up approach to construct a global seafood MeHg concentration dataset during 1995-2022. Firstly, it compiles a long-term time series marine-scale dataset of seafood MeHg concentrations, based on the reported seafood mercury concentrations from existing literature and machine learning methods. Subsequently, this study used the seafood catch volumes of each nation in different marine areas as weights to estimate the national-scale seafood MeHg concentrations. This dataset can provide essential data support for environmental impact assessment of mercury and its compounds as mentioned in Articles 12 and 19 of the Minamata Convention on Mercury.