Chen Lifeng, Liu Ruishan, Ye Haowei, Qian Jiajie, Lou Danfeng
Department of Clinical Engineering and Materials Supplies, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, People's Republic of China.
State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, People's Republic of China.
Infect Drug Resist. 2025 Feb 7;18:769-774. doi: 10.2147/IDR.S492203. eCollection 2025.
The global dissemination of carbapenem-resistant Enterobacteriaceae poses a significant threat to public health. In this study, we identified two clinical isolates, ACESH02628 and ACESH02857, harboring the gene in China. Both strains exhibited multidrug resistance, including reduced susceptibility to carbapenems, and carried transferable NDM-5-bearing plasmids. Specifically, S1-pulsed-field gel electrophoresis (PFGE), southern blotting, and whole-genome sequencing revealed that ACESH02628 contained an IncHI2/IncHI2A-type plasmid, while ACESH02857 carried an IncX3-type plasmid, both associated with the regional spread of NDM-5. Comparative analyses showed high genetic similarity with previously reported NDM-5 plasmids from and in China, underscoring the ease of horizontal transfer and potential for broader dissemination. Our findings highlight the emergence of NDM-5-producing of clinical origin and reinforce the need for vigilant surveillance and infection control measures to curb the proliferation of these highly resistant pathogens.
耐碳青霉烯类肠杆菌科细菌在全球范围内的传播对公共卫生构成了重大威胁。在本研究中，我们在中国鉴定出两株携带该基因的临床分离株ACESH02628和ACESH02857。这两株菌株均表现出多重耐药性，包括对碳青霉烯类药物敏感性降低，并携带可转移的携带NDM-5的质粒。具体而言，S1脉冲场凝胶电泳（PFGE）、Southern印迹和全基因组测序显示，ACESH02628含有一个IncHI2/IncHI2A类型的质粒，而ACESH02857携带一个IncX3类型的质粒，两者均与NDM-5的区域传播有关。比较分析表明，与中国此前报道的来自[具体地区1]和[具体地区2]的NDM-5质粒具有高度遗传相似性，强调了水平转移的易发性和更广泛传播的可能性。我们的研究结果凸显了临床来源的产NDM-5菌株的出现，并强化了进行警惕监测和感染控制措施以遏制这些高度耐药病原体扩散的必要性。
