Sugiyama H
Rev Infect Dis. 1979 Jul-Aug;1(4):683-8. doi: 10.1093/clinids/1.4.683.
Intestinal infection with Clostridium botulinum was produced by intragastric administration of C. botulinum spores in conventionally reared mice seven to 13 days old but not in younger or older mice. The 50% infective dose of one of the culture strains administered was 170 spores per nine-day-old mouse. Overt botulism did not develop in these animals, but infection with C. botulinum was evidenced by the presence of botulinal toxin in the colon for up to seven days after challenge. Infant mice were at least as sensitive to the lethal action of botulinal toxin as were adult mice, and evidence suggests that infant rats may have a similar age-related susceptibility to enteric botulinal infection. Germfree adult mice were very susceptible to infection with C. botulinum, acquiring intestinally infective doses of airborne spores. Within a few days after exposure to normal mice, the axenic mice became resistant to challenge with 10(5) C. botulinum spores.
通过给7至13日龄的常规饲养小鼠灌胃肉毒梭菌孢子可引发肠道肉毒梭菌感染，但年幼或年长的小鼠不会感染。所施用的一种培养菌株的50%感染剂量为每只9日龄小鼠170个孢子。这些动物未发生明显的肉毒中毒，但在攻击后长达7天的时间里，结肠中存在肉毒毒素证明感染了肉毒梭菌。幼鼠对肉毒毒素致死作用的敏感性至少与成年小鼠相同，有证据表明幼鼠对肠道肉毒梭菌感染可能具有类似的年龄相关易感性。无菌成年小鼠对肉毒梭菌感染非常敏感，可获得肠道感染剂量的空气传播孢子。在接触正常小鼠后的几天内，无菌小鼠对10(5)个肉毒梭菌孢子的攻击产生了抗性。
