McKee S A, Fyfe J A
Anal Biochem. 1985 Feb 1;144(2):429-31. doi: 10.1016/0003-2697(85)90136-8.
A method to measure orthophosphate which contaminates samples of ATP was developed. Concentrations of orthophosphate as low as 0.4% of the ATP concentration were determined using a zinc-molybdate reagent [D. A. Bencini, J. R. Wild, and G. A. O'Donovan, Anal. Biochem. 132, 254-258 (1983)] and continuous spectrophotometric monitoring of chromophore formation. Since the rate of ATP hydrolysis was pseudo-first order and was slow compared to the rate of chromophore formation, the initial concentration of phosphate could be readily determined by extrapolation to zero time. The method is rapid and reproducible, and requires a single, stable reagent.
开发了一种测量污染ATP样品的正磷酸盐的方法。使用锌钼酸盐试剂[D. A. 本奇尼、J. R. 怀尔德和G. A. 奥多诺万，《分析生物化学》132, 254 - 258 (1983)]并通过连续分光光度法监测发色团形成，可测定低至ATP浓度0.4%的正磷酸盐浓度。由于ATP水解速率为准一级反应，且与发色团形成速率相比很慢，因此通过外推至零时间可以很容易地确定磷酸盐的初始浓度。该方法快速且可重复，并且只需要一种稳定的试剂。
