Mu Chongyang, Feng Dawei, Khan Mashooq, Song Haoyang, Munir Sundas, Hu Qiongzheng, Yu Li
Key Laboratory of Colloid and Interface Chemistry, Ministry of Education, Shandong University, Jinan 250100, China.
Qilu University of Technology (Shandong Academy of Sciences), Shandong Analysis and Test Center, Jinan 250014, China.
Anal Chem. 2025 Feb 25;97(7):3926-3936. doi: 10.1021/acs.analchem.4c05166. Epub 2025 Feb 12.
Cholesteric liquid crystal elastomers (CLCEs) possess a unique characteristic that allows the manipulation of the reflected color by adjusting the spacing between layers. This attribute can create a reliable and cost-effective colorimetric sensing platform for the on-site detection of various substances. In this study, CLCE films were coupled with cross-linked poly(acrylic acid) (CLCE-PAA) or poly(dimethylaminoethyl methacrylate) (CLCE-PDA) films to monitor cholesterol and malathion levels, respectively. In both cases, the color change is recorded by a mobile phone camera, and the reflectance wavelength is measured spectrophotometrically. For on-site detection, a smartphone application was used to capture the film's image, process the color data into hue () values, and calculate the corresponding concentration of the tested analyte via an analysis program. Cholesterol and malathion can be detected within a linear range of 0.2-1.0 mM and 1-10,000 ng/mL, respectively. The corresponding recoveries for actual sample analysis were 86-115% and 87-122% for cholesterol and malathion, respectively. This system offers a practical solution for on-site testing of cholesterol and malathion in biological and environmental samples.
胆甾相液晶弹性体（CLCEs）具有一种独特特性，即可以通过调节层间间距来控制反射颜色。这一属性能够创建一个可靠且经济高效的比色传感平台，用于现场检测各种物质。在本研究中，CLCE薄膜分别与交联聚丙烯酸（CLCE - PAA）或聚甲基丙烯酸二甲氨基乙酯（CLCE - PDA）薄膜相结合，以监测胆固醇和马拉硫磷的水平。在这两种情况下，颜色变化都由手机摄像头记录，反射波长通过分光光度法测量。对于现场检测，使用智能手机应用程序拍摄薄膜图像，将颜色数据处理为色调（）值，并通过分析程序计算被测分析物的相应浓度。胆固醇和马拉硫磷的检测线性范围分别为0.2 - 1.0 mM和1 - 10,000 ng/mL。实际样品分析中胆固醇和马拉硫磷的相应回收率分别为86 - 115%和87 - 122%。该系统为生物和环境样品中胆固醇和马拉硫磷的现场检测提供了一种实用解决方案。
