Yuksel Aysun, Celayir Damla Nur, Yenilmez Tunoglu Ezgi Nurdan, Tutar Lütfi, Tutar Yusuf
Department of Nutrition and Dietetics, Medeniyet University, Istanbul 34720, Türkiye.
Department of Nutrition and Dietetics, University of Health Sciences, Istanbul 34668, Türkiye.
Nutrients. 2025 Jan 21;17(3):374. doi: 10.3390/nu17030374.
BACKGROUND/OBJECTIVES: (Kenger) is a traditional medicinal plant and exhibits potential anticancer properties. This study investigates the cytotoxic and apoptotic effects of its water extract on human lung carcinoma A549 cells.
A lung cancer cell line was treated with extract. The metabolic content of the extract that plays key roles in anticancer was detected by high-performance liquid chromatography. Anticancer properties were further detected by a flow cytometer apoptosis assay, and signaling pathways were determined by a PCR array through hub gene expression alteration. Gene enrichment analysis and network pharmacology correlated metabolites and pathways that were involved in anticancer effects.
The metabolite content of was analyzed, and gallic acid, clorogenic acid, hydroxybenzoic acid, caffeic acid, epicatechin, p-coumaric acid, salicylic acid, apigenin 7 glucoside, and cinnamic acid were detected as key compounds. Lung cancer cell line A549 was treated with the extract at increasing concentrations for 24, 48, and 72 h, and its effects on cell viability were determined by MTT analysis. A statistically significant difference was observed for IC concentrations depending on incubation times. It was also observed that the water extract significantly increased apoptosis in A549 cells in comparison with the control group. extract's effect on lung cancer cell line was measured using the signal pathway PCR array gene set. Gene enrichment analysis of the array expression data confirmed activation of apoptosis-related pathways, particularly the upregulation of BAX and downregulation of HSP90.
These findings suggest that metabolites provide promising selective anticancer drug candidates and potential drug templates to prevent side effects and resistance of current clinical drug treatments.
背景/目的：（肯格尔）是一种传统药用植物，具有潜在的抗癌特性。本研究调查了其水提取物对人肺癌A549细胞的细胞毒性和凋亡作用。
用提取物处理肺癌细胞系。通过高效液相色谱法检测提取物中在抗癌中起关键作用的代谢成分。通过流式细胞仪凋亡检测进一步检测抗癌特性，并通过PCR阵列通过枢纽基因表达改变确定信号通路。基因富集分析和网络药理学关联了参与抗癌作用的代谢物和通路。
分析了提取物的代谢物含量，检测到没食子酸、绿原酸、羟基苯甲酸、咖啡酸、表儿茶素、对香豆酸、水杨酸、芹菜素7-葡萄糖苷和肉桂酸为关键化合物。用不同浓度的提取物处理肺癌细胞系A549 24、48和72小时，通过MTT分析确定其对细胞活力的影响。观察到IC浓度因孵育时间而异，具有统计学显著差异。还观察到，与对照组相比，提取物水提取物显著增加了A549细胞的凋亡。使用信号通路PCR阵列基因集测量提取物对肺癌细胞系的作用。对阵列表达数据的基因富集分析证实了凋亡相关通路的激活，特别是BAX的上调和HSP90的下调。
这些发现表明，提取物的代谢物提供了有前景的选择性抗癌药物候选物和潜在的药物模板，以预防当前临床药物治疗的副作用和耐药性。
