Rozmus Dominika, Fiedorowicz Ewa, Płomiński Janusz, Cieślińska Anna
Department of Biochemistry, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland.
Orthopaedic and Trauma Teaching Hospital, 05-400 Otwock, Poland.
Nutrients. 2025 Jan 21;17(3):378. doi: 10.3390/nu17030378.
Total hip replacement (THR) significantly improves patients' quality of life; however, prosthesis loosening remains a significant complication. Vitamin D, essential for calcium homeostasis and bone mineralization, is transported and stabilized by vitamin D binding protein (VDBP). Common single nucleotide polymorphisms (SNPs) in the VDBP gene, rs4588 and rs7041, may influence serum vitamin D levels and potentially impact THR outcomes. This study aimed to analyze the association between these SNPs, serum levels of VDBP and 25(OH)D, and their potential roles in THR outcomes. The study included three patient groups: (1) patients undergoing arthroscopy after a THR without prosthesis loosening (CA-Control Arthroplasty), (2) patients with hip prosthesis loosening (L-Loosening), and (3) a control group (C-Control). Genotyping of rs4588 and rs7041 in the VDBP gene was conducted using PCR-RFLP and TaqMan Genotyping real-time PCR. Serum levels of VDBP and 25(OH)D were measured using ELISA. Comparisons between groups were performed using statistical analyses, including odds ratios (OR) and significance testing (-values). There are significant differences in VDBP concentrations between the groups: L vs. CA ( < 0.0001), L vs. C ( = 0.0118), L vs. L + CA ( = 0.0013), CA vs. C ( < 0.0001), and CA vs. L + CA ( < 0.0001), and in 25(OH)D concentrations between groups: L vs. C ( < 0.0001), CA vs. C ( = 0.0008), and C vs. L + CA ( < 0.0001). The study findings suggest a protective role of 25(OH)D against prosthesis loosening in THR. The rs4588 SNP in the VDBP gene may increase the risk of loosening, while differences in VDBP and 25(OH)D concentrations between patient groups highlight their potential importance in THR outcomes.
全髋关节置换术（THR）显著提高了患者的生活质量；然而，假体松动仍然是一个重大并发症。维生素D对钙稳态和骨矿化至关重要，它由维生素D结合蛋白（VDBP）运输和稳定。VDBP基因中的常见单核苷酸多态性（SNP），即rs4588和rs7041，可能会影响血清维生素D水平，并可能影响THR的结果。本研究旨在分析这些SNP、VDBP和25（OH）D的血清水平之间的关联，以及它们在THR结果中的潜在作用。该研究包括三个患者组：（1）THR后未发生假体松动接受关节镜检查的患者（CA-对照关节成形术），（2）髋关节假体松动的患者（L-松动），以及（3）一个对照组（C-对照）。使用PCR-RFLP和TaqMan基因分型实时PCR对VDBP基因中的rs4588和rs7041进行基因分型。使用ELISA测量VDBP和25（OH）D的血清水平。使用包括优势比（OR）和显著性检验（P值）在内的统计分析进行组间比较。各组之间的VDBP浓度存在显著差异：L与CA（P<0.0001），L与C（P = 0.0118），L与L + CA（P = 0.0013），CA与C（P<0.0001），以及CA与L + CA（P<0.0001），并且各组之间的25（OH）D浓度也存在显著差异：L与C（P<0.0001），CA与C（P = 0.0008），以及C与L + CA（P<0.0001）。研究结果表明，25（OH）D在THR中对假体松动具有保护作用。VDBP基因中的rs4588 SNP可能会增加松动风险，而患者组之间VDBP和25（OH）D浓度的差异突出了它们在THR结果中的潜在重要性。
