包含菊科植物精油的纳米技术制剂：利什曼原虫杀灭和抗炎治疗的环保方法

Nanotechnological Formulation Incorporating (Asteraceae) Essential Oil: An Ecofriendly Approach for Leishmanicidal and Anti-Inflammatory Therapy.

作者信息

Marques Estela Mesquita, Santos Andrade Lucas George, Rebelo Alencar Luciana Magalhães, Dias Rates Erick Rafael, Ribeiro Rachel Melo, Carvalho Rafael Cardoso, de Souza Nunes Glécilla Colombelli, Sara Lopes Lera-Nonose Daniele Stéfanie, Gonçalves Maria Julia Schiavon, Lonardoni Maria Valdrinez Campana, Souza Melissa Pires, Costa Emmanoel Vilaça, Gonçalves Renato Sonchini

机构信息

Laboratory of Chemistry of Natural Products, Department of Chemistry, Federal University of Maranhão (UFMA), São Luís 65080-805, Brazil.

Laboratory of Biophysics and Nanosystems, Department of Physics, Federal University of Maranhão, São Luís 65080-805, Brazil.

出版信息

Polymers (Basel). 2025 Jan 30;17(3):379. doi: 10.3390/polym17030379.

DOI:10.3390/polym17030379

PMID:39940581

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11820061/

Abstract

Cutaneous leishmaniasis caused by is a significant public health issue. This study aimed to evaluate an ecofriendly, thermosensitive nanogel, developed using a low-energy, solvent-free method, incorporating F127 and Carbopol 974P copolymers, and enriched with essential oil (EO) for its leishmanicidal and anti-inflammatory properties. The nanogel was prepared and characterized through FTIR, DLS, SEM, and AFM to confirm the incorporation of EO as well as its stability and rheological properties. In vitro leishmanicidal activity was evaluated on promastigotes, and in vivo anti-inflammatory effects were assessed using a rat paw edema model. In vitro, nGF3 (EO-loaded nanogel) demonstrated significant leishmanicidal activity, with promastigote mortality rates exceeding 80% at 24 h and 90% at 48 h. In vivo, nGF1, nGF2, and nGF3 exhibited anti-inflammatory effects, with nGF2 and nGF3 reducing edema by 62.7% at 2 h post-treatment. The empty nanogel (nGF0) showed minimal anti-inflammatory activity. The ecofriendly EO-loaded nanogel (nGF3) demonstrated strong leishmanicidal and anti-inflammatory effects, presenting a promising candidate for cutaneous leishmaniasis treatment. Further studies are necessary to explore its clinical potential.

摘要

由[病原体名称未给出]引起的皮肤利什曼病是一个重大的公共卫生问题。本研究旨在评估一种采用低能量、无溶剂方法开发的环保型热敏纳米凝胶，该纳米凝胶包含F127和卡波姆974P共聚物，并富含[精油名称未给出]精油（EO），以研究其杀利什曼原虫和抗炎特性。通过傅里叶变换红外光谱（FTIR）、动态光散射（DLS）、扫描电子显微镜（SEM）和原子力显微镜（AFM）对纳米凝胶进行制备和表征，以确认EO的掺入及其稳定性和流变学性质。在体外对前鞭毛体进行杀利什曼原虫活性评估，并使用大鼠足肿胀模型评估体内抗炎作用。在体外，nGF3（负载EO的纳米凝胶）表现出显著的杀利什曼原虫活性，在前鞭毛体死亡率方面，24小时时超过80%，48小时时超过90%。在体内，nGF1、nGF2和nGF3表现出抗炎作用，nGF2和nGF3在治疗后2小时使肿胀减少62.7%。空纳米凝胶（nGF0）显示出最小的抗炎活性。这种环保型负载EO的纳米凝胶（nGF3）表现出强大的杀利什曼原虫和抗炎作用，是治疗皮肤利什曼病的一个有前景的候选物。有必要进一步研究以探索其临床潜力。