Kim Ji-Hee, Park Bog-Im, Kim Young-Hoi, Yoon Ji-Su, Choi Na-Young, Kim Kang-Ju
Department of Convergence Technology for Food Industry, School of Food, Wonkwang University, Iksan 54538, Jeonbuk, Republic of Korea.
Transdisciplinary Major in Learning Health Systems, Department of Health and Safety Convergence Science, Graduate School, Korea University, Seoul 02841, Republic of Korea.
Molecules. 2025 Jan 25;30(3):553. doi: 10.3390/molecules30030553.
The essential oil extracted from the flowers of var. (Maxim.) Kitam (CZEO), family Asteraceae, was investigated to determine its ability to inhibit the pathogenicity of methicillin-resistant (MRSA). The chemical composition of CZEO was analyzed using gas chromatography-flame ionization detector and gas chromatography-mass spectrometry, and 88 compounds were identified and categorized as monoterpenes (68.82%), sesquiterpenes (17.82%), and others (5.01%). CZEO inhibited MRSA floating cell growth, acid production, and biofilm formation in a concentration-dependent manner. Furthermore, confocal laser scanning and scanning electron microscopy confirmed that the CZEO treatment decreased MRSA viability and notably reduced the three-dimensional density of the biofilm. Real-time PCR demonstrated that the mRNA expression of the MRSA gene A (), accessory gene regulator A (), staphylococcal accessory regulator A (), and staphylococcal enterotoxin A (), which are pivotal genes implicated in MRSA pathogenicity, declined in a concentration-dependent manner following the CZEO treatment compared with the control. Thus, CZEO appeared to directly target the pathogenicity MRSA regulators. These findings substantiate the potential of CZEO as a natural antimicrobial agent for preventing MRSA infections.
对菊科植物北苍术（Atractylodes chinensis (DC.) Koidz. var. (Maxim.) Kitam (CZEO)）花朵中提取的精油进行了研究，以确定其抑制耐甲氧西林金黄色葡萄球菌（MRSA）致病性的能力。使用气相色谱 - 火焰离子化检测器和气相色谱 - 质谱联用仪分析了CZEO的化学成分，鉴定出88种化合物，并将其分类为单萜类（68.82%）、倍半萜类（17.82%）和其他类（5.01%）。CZEO以浓度依赖的方式抑制MRSA浮游细胞生长、产酸和生物膜形成。此外，共聚焦激光扫描显微镜和扫描电子显微镜证实，CZEO处理降低了MRSA的活力，并显著降低了生物膜的三维密度。实时聚合酶链反应表明，与对照相比，CZEO处理后，MRSA基因A（）、辅助基因调节因子A（）、葡萄球菌辅助调节因子A（）和葡萄球菌肠毒素A（）的mRNA表达以浓度依赖的方式下降，这些基因是与MRSA致病性相关的关键基因。因此，CZEO似乎直接靶向MRSA致病性调节因子。这些发现证实了CZEO作为预防MRSA感染的天然抗菌剂的潜力。
