Čechovičienė Indrė, Tarasevičienė Živilė, Hallman Ewelina, Jabłońska-Trypuć Agata, Česonienė Laima, Šileikienė Daiva
Department of Plant Biology and Food Sciences, Agriculture Academy Vytautas Magnus University, Donelaičio Str. 58, 44248 Kaunas, Lithuania.
Department of Functional and Organic Food, Institute of Human Nutrition Sciences, Warsaw University of Life Sciences, Nowoursynowska Str. 159C, 02-776 Warsaw, Poland.
Plants (Basel). 2025 Jan 27;14(3):384. doi: 10.3390/plants14030384.
Blackberries are seasonal berries that are processed into various products leaving a large amount of residues after processing, and therefore the most effective ways of utilising the residues need to be evaluated. The aim of this study was to determine the effect of different extraction methods on the chemical content of blackberry pomace extracts from different cultivars and their effect on the viability of the Caco-2 colorectal adenocarcinoma cell line and CCD-18Co normal colon fibroblast cancer cells. Blackberry pomace from berries of the cultivars 'Polar', 'Orkan', and 'Brzezina' was extracted by ultrasound-assisted extraction (UAE), microwave-assisted extraction (MAE) and a combination of these two extraction methods (MAE+UAE). The phenolic profile and the amount of organic acids and sugars were detected by HPLC. The spectrophotometric method was used to determine the amount of total phenolics, total flavonoids, and total anthocyanins. The cytotoxicity of the extracts was measured by the MTT assay. The chemical content of the extracts depends on the blackberry cultivar, the extraction method, and its interaction. Only the DPPH antioxidant activity did not depend on these factors and had no statistically significant differences between the different extracts. The extracts at a concentration of 5.0% increased the growth of both cancer cells, while the extracts at 1% and 2.5%, depending on the cultivar, reduced the growth of these cells. The MAE and UAE extracts of the 'Orkan' cultivar at concentrations of 1%, 1.5%, 2%, and 2.5% best inhibited the viability of Caco-2 cells. The extracts inhibited the growth of the Caco-2 cell line better than CCD-18Co normal colon fibroblasts.
黑莓是季节性浆果，经加工制成各种产品后会产生大量残渣，因此需要评估利用这些残渣的最有效方法。本研究的目的是确定不同提取方法对不同品种黑莓果渣提取物化学成分的影响，以及它们对Caco-2结肠腺癌细胞系和CCD-18Co正常结肠成纤维细胞活力的影响。采用超声辅助提取（UAE）、微波辅助提取（MAE）以及这两种提取方法的组合（MAE+UAE）对‘Polar’、‘Orkan’和‘Brzezina’品种黑莓的果渣进行提取。通过高效液相色谱法检测酚类物质谱、有机酸和糖的含量。采用分光光度法测定总酚、总黄酮和总花青素的含量。通过MTT法测定提取物的细胞毒性。提取物的化学成分取决于黑莓品种、提取方法及其相互作用。只有DPPH抗氧化活性不依赖于这些因素，不同提取物之间无统计学显著差异。浓度为5.0%的提取物促进了两种癌细胞的生长，而浓度为1%和2.5%的提取物，因品种而异，抑制了这些细胞的生长。‘Orkan’品种浓度为1%、1.5%、2%和2.5%的MAE和UAE提取物对Caco-2细胞活力的抑制效果最佳。提取物对Caco-2细胞系生长的抑制作用优于CCD-18Co正常结肠成纤维细胞。
