转录组分析为黄尾鰤（Seriola dumerili）卵母细胞生长和成熟提供了新见解。

Transcriptomic Analysis Provides New Insights into Oocyte Growth and Maturation in Greater Amberjack ().

作者信息

Yang Jiahui, Ru Xiaoying, Huang Yang, Wu Jinhui, Yang Tonglin, Chen Peipei, Li Jin, Zhu Kunfeng, Zhu Chunhua

机构信息

Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Fisheries College of Guangdong Ocean University, Zhanjiang 524088, China.

Development and Research Center for Biological Marine Resources, Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Zhanjiang 524025, China.

出版信息

Animals (Basel). 2025 Jan 24;15(3):333. doi: 10.3390/ani15030333.

DOI:10.3390/ani15030333

PMID:39943103

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11815777/

Abstract

The greater amberjack () is an emerging marine fish that is increasingly favored in aquaculture. Currently, there are few studies on the development and regulation of greater amberjack ovaries. In this study, the ovary transcriptome profiles of greater amberjack at three different stages (stage II, III, and IV) were performed, and identified the genes and pathways that may play significant roles in the processes of follicle growth and maturation. A total of 6597, and 1061 differentially expressed genes (DEGs) were detected in FII vs. FIII, FIII vs. FIV, and FII vs. FIV stages, respectively. GO and KEGG enrichment analyses revealed that these DEG are primarily involved in steroid hormone biosynthesis (e.g., , , , , ), lipid metabolism (e.g., , , and ), and meiotic arrest and resumption (e.g., , , , , , , ). Additionally, several signaling pathways involved in ovarian development have been identified, including the PI3K-Akt, Wnt, TGF-beta, GnRH, and immune-related signaling pathways. qPCR results of nine representative genes related to steroid hormone synthesis and cell growth verified the reliability of the generated RNA-seq data. This research contributes to our comprehension of the molecular processes underlying ovarian growth and maturation in marine fishes and provides a theoretical basis for the investigation of functional genes associated with oogenesis in greater amberjack.

摘要

黄尾鰤（）是一种新兴的海洋鱼类，在水产养殖中越来越受到青睐。目前，关于黄尾鰤卵巢发育和调控的研究较少。在本研究中，对黄尾鰤三个不同阶段（II期、III期和IV期）的卵巢转录组图谱进行了分析，并确定了可能在卵泡生长和成熟过程中发挥重要作用的基因和通路。在FII与FIII、FIII与FIV以及FII与FIV阶段分别检测到6597个和1061个差异表达基因（DEG）。GO和KEGG富集分析表明，这些DEG主要参与类固醇激素生物合成（如，，，，）、脂质代谢（如，，和）以及减数分裂停滞和恢复（如，，，，，，）。此外，还确定了几条参与卵巢发育的信号通路，包括PI3K-Akt、Wnt、TGF-β、GnRH和免疫相关信号通路。九个与类固醇激素合成和细胞生长相关的代表性基因的qPCR结果验证了所生成的RNA-seq数据的可靠性。本研究有助于我们理解海洋鱼类卵巢生长和成熟的分子过程，并为研究黄尾鰤卵子发生相关功能基因提供理论依据。