Quentmeier A, Antranikian G
Arch Microbiol. 1985 Feb;141(1):85-90. doi: 10.1007/BF00446745.
Cells of Clostridium sporosphaeroides which were grown on citrate contained citrate lyase and citrate lyase acetylating enzyme, but no detectable citrate synthase and citrate lyase deacetylase activities. Citrate lyase from C. sporosphaeroides was purified to homogeneity as judged by polyacrylamide gel electrophoresis and high performance liquid chromatography. In contrast to the enzyme from Clostridium sphenoides, the addition of L-glutamate was not necessary for activity and stabilization of the enzyme. The purified enzyme had a specific activity of 34 U/mg protein and was comparable to other citrate lyases with respect to its molecular weight and subunit composition. Electron microscopic investigations showed that similar to the lyase from C. sphenoides and in contrast to all other citrate lyases examined so far, the majority of the enzyme molecules was present in "star" form.
在柠檬酸盐上生长的球形芽孢梭菌细胞含有柠檬酸裂解酶和柠檬酸裂解酶乙酰化酶，但未检测到柠檬酸合酶和柠檬酸裂解酶脱乙酰酶活性。通过聚丙烯酰胺凝胶电泳和高效液相色谱判断，来自球形芽孢梭菌的柠檬酸裂解酶被纯化至同质。与来自梭状芽孢杆菌的酶相反，添加L-谷氨酸对于该酶的活性和稳定性不是必需的。纯化后的酶比活性为34 U/mg蛋白质，就其分子量和亚基组成而言，与其他柠檬酸裂解酶相当。电子显微镜研究表明，与来自梭状芽孢杆菌的裂解酶类似，与迄今为止检测的所有其他柠檬酸裂解酶相反，大多数酶分子以“星形”形式存在。
