Assessment of Anticancer and Antimicrobial Potential of Bioactive Metabolites and Optimization of Culture Conditions of PB-St2 for High Yields.

The following study aimed to characterize the biological potential of the purified compounds of PB-St2. Optimization of temperature and incubation time of 32oC and 72 h yielded the highest crude extract weight and optical density of bacterial culture. HPLC analysis of the crude metabolite extract (purified using gravitational column chromatography) showed three fractions named as PC1, PC2, and PC3. HPLC-purified fractions were subjected to LC-MS/MS analysis and the data was compared using reference library. Fraction PC1 was identified as mupirocin, PC2 as phenazine-1-carboxylic acid (PCA), and PC3 as the mixture of three compounds including pyoluteorin, PCA and 2-hydroxyphenazine (2-OH-phz). Fungicidal potential of the purified compounds was assessed against phytopathogens including , , , and . Fraction PC3 showed the highest fungicidal activity of 89%, whereas, the least antifungal activity (27%) was noted for mupirocin. Antibacterial activity of the purified compounds against Gram-positive pathogen , and Gram-negative pathogens , , and was also assessed. Fraction PC3 demonstrated the highest antibacterial activity against and showing 1.8 cm, and 0.9 cm zones of inhibition, respectively. Against and , the antibacterial activity of PB-St2 crude extract was slightly higher than the fraction PC3. The fraction PC3 also demonstrated the highest IC against HepG-2 and SF767 cancer cell lines at 25 μg and 20 μg concentrations, respectively. The multifaceted attributes of PB-St2 make it an ideal candidate for agricultural and pharmacological applications.