Rossant J, Papaioannou V E
Exp Cell Res. 1985 Jan;156(1):213-20. doi: 10.1016/0014-4827(85)90275-7.
Early embryonic cells are capable of regulating the proliferation of some embryonal carcinoma (EC) cells in vivo although other EC lines are not affected. Up to 80% of fetuses are chimeric following injection of P10EC cells into blastocysts and half of these are normal. P19, on the other hand, is not controlled by the embryo and all chimeric fetuses are abnormal. P10 and P19 cells were injected into blastocysts and vesicles of pure trophectoderm and examined following culture. EC cells grew from 62/72 P19-injected blastocysts but from only 7/73 P10-injected blastocysts. Both cell lines grew from injected trophectoderm vesicles in culture: P19 grew from 10/17 injected vesicles and P10 grew from 9/13 injected vesicles, suggesting that trophectoderm alone is not responsible for controlling EC growth. When EC-injected vesicles were transferred to foster mothers, normal development did not ensue, indicating the failure of the trophectoderm to normalize EC cells in vivo. Results indicate that EC-embryo combinations that allow EC outgrowth in vitro are also those that result in incomplete control of EC proliferation in chimeras and that the in vitro system can thus be used to investigate factors in the embryonic environment that control EC proliferation and differentiation.
早期胚胎细胞能够在体内调节某些胚胎癌细胞（EC）的增殖，尽管其他EC细胞系不受影响。将P10EC细胞注射到囊胚中后，高达80%的胎儿会出现嵌合体，其中一半是正常的。另一方面，P19不受胚胎控制，所有嵌合胎儿都是异常的。将P10和P19细胞注射到纯滋养外胚层的囊胚和囊泡中，并在培养后进行检查。EC细胞从62/72个注射了P19的囊胚中生长出来，但仅从7/73个注射了P10的囊胚中生长出来。两种细胞系在培养中都从注射的滋养外胚层囊泡中生长：P19从10/17个注射的囊泡中生长，P10从9/13个注射的囊泡中生长，这表明仅滋养外胚层并不负责控制EC生长。当将注射了EC的囊泡转移到代孕母亲体内时，并未出现正常发育，这表明滋养外胚层在体内无法使EC细胞正常化。结果表明，在体外允许EC生长的EC-胚胎组合，也是那些在嵌合体中导致对EC增殖控制不完全的组合，因此体外系统可用于研究胚胎环境中控制EC增殖和分化的因素。
