Pieri C, Muzzioli M, Giuli C, Bertoni-Freddari C
Boll Soc Ital Biol Sper. 1985 Feb 28;61(2):175-81.
The present study was aimed to ascertain whether Na+ influx can be involved in regulation of blastogenesis and proliferation of PHA stimulated mouse splenic lymphocytes. The cells were cultivated in the presence of different concentrations of amiloride, an inhibitor of passive Na+ influx, and cellular activation was monitored by 3H-thymidine incorporation and blast index determination. The drug was not toxic and inhibited cell growth in concentrations ranging from 1 X 10(-3) to 1 X 10(-5) mmol/ml. The results are in agreement with the hypothesis that Na influx is necessary for PHA evoked mouse splenic lymphocyte activation.
本研究旨在确定Na+内流是否参与调节PHA刺激的小鼠脾淋巴细胞的母细胞化和增殖。细胞在不同浓度的氨氯吡咪（一种被动Na+内流抑制剂）存在下培养，并通过3H-胸腺嘧啶核苷掺入和母细胞指数测定来监测细胞活化。该药物无毒，在1×10(-3)至1×10(-5) mmol/ml的浓度范围内抑制细胞生长。结果与Na+内流是PHA诱导小鼠脾淋巴细胞活化所必需的这一假设一致。
