Derayea Sayed M, Madian Huda, Samir Ebtihal, Badr-Eldin Khaled M
Analytical Chemistry Department, Faculty of Pharmacy, Minia University, Minia, 61519, Egypt.
Pharmaceutical Analytical Chemistry Department, Deraya University, New Minia, 61519, Minia, Egypt.
BMC Chem. 2025 Feb 13;19(1):38. doi: 10.1186/s13065-025-01398-1.
The present study was dedicated for solving the challenge of the overlapped spectra of olanzapine (OLZ) and fluoxetine (FLX) for their concurrent, accurate and precise determination without prior physical separation. The developed methods had to venture away from those that depend on chromatography, because of their negative effects on the environment due to using hazardous organic solvents as well as their high cost. The suggested methods were based on spectrophotometry combined with simple mathematical treatments of the spectra. These approaches are simple, rapid, inexpensive and reliable options for the effective assay of FLX and OLZ in pharmaceutical dosage forms. Ratio subtraction method was devoted for estimation of both drugs, while dual wavelength method and absorptivity factor method could be utilized in the estimation of FLX and OLZ, respectively. All the suggested methods were efficiently applied for the determination of the studied drugs in laboratory prepared mixtures and their pharmaceutical formulations. The environmental safety of the involved procedures was assessed by applying the Eco Score scale and complex modified GAPI methods. The methods were proved to be highly safe since no large volume of solvents were used, and no derivatizing reagent or drastic experimental conditions were involved. In addition, the procedures consume little energy and produce a small amount of waste. In conclusion, for FLX determination, the use of dual wavelength method is preferable because it needs very low mathematical treatment than the ratio subtraction method. However, for OLZ, the ratio subtraction method has higher sensitivity than absorptivity factor method although the latter is simpler.
本研究致力于解决奥氮平（OLZ）和氟西汀（FLX）光谱重叠的问题，以便在无需预先物理分离的情况下同时、准确且精确地测定它们。由于使用有害有机溶剂对环境有负面影响且成本高昂，所开发的方法必须摒弃依赖色谱法的方法。建议的方法基于分光光度法并结合对光谱的简单数学处理。这些方法对于有效测定药物剂型中的FLX和OLZ而言，是简单、快速、廉价且可靠的选择。比率减法用于估算两种药物，而双波长法和吸光系数法可分别用于估算FLX和OLZ。所有建议的方法都有效地应用于测定实验室制备的混合物及其药物制剂中的研究药物。通过应用生态评分量表和复杂的改良GAPI方法评估了所涉程序的环境安全性。由于未使用大量溶剂，且未涉及衍生试剂或剧烈的实验条件，这些方法被证明具有高度安全性。此外，这些程序消耗的能量少且产生的废物量小。总之，对于FLX的测定，使用双波长法更可取，因为它比分率减法所需的数学处理要少得多。然而，对于OLZ，比率减法比吸光系数法具有更高的灵敏度，尽管后者更简单。
