Hong Yiqin, Wang Hui, Xie Hanyan, Zhong Xinyi, Chen Xu, Yu Lishuang, Zhang Yawen, Zhang Jingmei, Wang Qiyan, Tang Binghua, Lu Linghui, Guo Dongqing
School of Life Sciences, Beijing University of Chinese Medicine, Beijing 100029, China.
School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.
Chin Herb Med. 2024 Apr 30;17(1):139-147. doi: 10.1016/j.chmed.2023.12.007. eCollection 2025 Jan.
Therapeutic angiogenesis has become a promising approach for treating ischemic heart disease (IHD). The present study aims to investigate the effects of Qishen Granule (QSG) on angiogenesis in myocardial ischemia (MI) and the potential mechanism.
study was conducted on rat model of myocardial infarction. QSG was performed daily at a dose of 2.352 g/kg for four weeks. Cardiac function was assessed by echocardiogram and pro-angiogenic effects were evaluated by Laser Doppler and CD31 expression. Oxygen-glucose deprivation (OGD) was applied in cultured human umbilical vein endothelial cells (HUVECs). Cell viability, wound healing and tube formation assay were used to test functions of HUVECs. ELISA and Western blots were used to assess protein expressions of bone morphogenetic protein 2-delta-like 4-notch homolog 1 (BMP2-Dll4-Notch1) signaling pathway.
The results showed that QSG improved heart function, cardiac blood flow and microvessel density in myocardial ischemic rats. QSG protected HUVECs by promoting the cell viability and tube formation. QSG upregulated bone morphogenetic protein-2 (BMP2) and downregulated delta-like 4 (Dll4) and notch homolog 1 (Notch1) expressions both in rats and HUVECs.
QSG protected against MI by promoting angiogenesis through BMP2-Dll4-Notch1 pathway. BMP2 might be a promising therapeutic target for IHD.
治疗性血管生成已成为治疗缺血性心脏病（IHD）的一种有前景的方法。本研究旨在探讨芪参颗粒（QSG）对心肌缺血（MI）血管生成的影响及其潜在机制。
以大鼠心肌梗死模型进行研究。QSG以2.352 g/kg的剂量每日给药，持续四周。通过超声心动图评估心脏功能，通过激光多普勒和CD31表达评估促血管生成作用。在培养的人脐静脉内皮细胞（HUVECs）中应用氧葡萄糖剥夺（OGD）。采用细胞活力、伤口愈合和管腔形成试验检测HUVECs的功能。采用酶联免疫吸附测定（ELISA）和蛋白质免疫印迹法评估骨形态发生蛋白2-δ样4-Notch同源物1（BMP2-Dll4-Notch1）信号通路的蛋白表达。
结果显示，QSG改善了心肌缺血大鼠的心脏功能、心脏血流量和微血管密度。QSG通过促进细胞活力和管腔形成保护HUVECs。QSG上调大鼠和HUVECs中骨形态发生蛋白-2（BMP2）的表达，下调δ样4（Dll4）和Notch同源物1（Notch1）的表达。
QSG通过BMP2-Dll4-Notch1通路促进血管生成，从而保护心肌梗死。BMP2可能是IHD一个有前景的治疗靶点。
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