氯丙嗪对棕色固氮菌膜中电子传递的抑制作用。

Chlorpromazine inhibition of electron transport in Azotobacter vinelandii membranes.

作者信息

Wong T Y, Maier R J

出版信息

Biochim Biophys Acta. 1985 May 31;807(3):320-3. doi: 10.1016/0005-2728(85)90264-6.

Abstract

Chlorpromazine was a potent inhibitor of O2-dependent malate oxidation, but not of H2 oxidation in Azotobacter vinelandii membranes. However, chlorpromazine did not significantly affect the activity of malate reductase or the reduction of cytochromes c and d. In the presence of chlorpromazine, cytochrome o failed to form a complex with CO. The site of action of chlorpromazine seems to be in the cytochromes c to cytochrome o branch, the pathway utilized by malate, succinate and NADH, but not by H2.

摘要

氯丙嗪是维涅兰德固氮菌膜中依赖氧气的苹果酸氧化的强效抑制剂，但不是氢气氧化的抑制剂。然而，氯丙嗪对苹果酸还原酶的活性或细胞色素c和d的还原没有显著影响。在氯丙嗪存在的情况下，细胞色素o无法与一氧化碳形成复合物。氯丙嗪的作用位点似乎位于细胞色素c到细胞色素o的分支中，这是苹果酸、琥珀酸和NADH所利用的途径，但氢气不利用该途径。