5,6-二氯苯并咪唑-1-β-D-核糖苷，烟草细胞悬浮培养中细胞分裂素活性的特异性抑制剂。

5,6-Dichlorobenzimidazole-1-beta-D-riboside, specific inhibitor of cytokinin activity in tobacco cell suspension cultures.

作者信息

Aké S, Péaud-Lenoël C

出版信息

Biochimie. 1985 Jan;67(1):155-60. doi: 10.1016/s0300-9084(85)80243-1.

Abstract

Tobacco cell suspension cultures were supplemented with 4 to 10 microM 5,6-dichloro-1-beta-D-ribofuranosyl-benzimidazole (DBR). The cell suspensions were cultured in the presence or without a cytokinin, kinetin (N6-furfuryladenine). The responses of the cultures to DRB and (or) kinetin were measured by the evolution of cell mass or cell counts in the cultures, as a function of time or kinetin concentration. Chlorophyll biosynthesis was also used as a test-response to cytokinin and (or) DRB activities. It was concluded that DRB behaves as a specific inhibitor of the physiological responses to cytokinins. The evolution of the inhibition ratio versus kinetin concentration shows that DRB is not a competitor of the cytokinins.

摘要

烟草细胞悬浮培养物中添加了4至10微摩尔的5,6-二氯-1-β-D-呋喃核糖基苯并咪唑（DBR）。细胞悬浮液在有或没有细胞分裂素激动素（N6-糠基腺嘌呤）的情况下进行培养。通过培养物中细胞质量或细胞计数随时间或激动素浓度的变化来测量培养物对DBR和（或）激动素的反应。叶绿素生物合成也被用作对细胞分裂素和（或）DBR活性的测试反应。得出的结论是，DBR表现为细胞分裂素生理反应的特异性抑制剂。抑制率随激动素浓度的变化表明，DBR不是细胞分裂素的竞争者。